Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

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5.9K Views

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09:08 min

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December 22nd, 2020

DOI :

10.3791/62008-v

December 22nd, 2020

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Kelly Veerasammy*¹^,², Yuki X. Chen*¹^,², Sami Sauma*¹, Mathilde Pruvost¹, David K. Dansu¹, Tenzin Choetso¹^,², Tiffany Zhong³, Damien Marechal¹, Patrizia Casaccia¹, Rinat Abzalimov⁴^,⁵, Ye He¹^,⁵

¹The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York, ²The City College of New York, CUNY, ³The Bronx High School of Science, ⁴The Graduate Center - Advanced Science Research Center, Structural Biology Initiative, The City University of New York, ⁵The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York

Podsumowanie

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Indium Tin Oxide Slides

Cryo Tissue Embedding

Rozdziały w tym wideo

0:04

Introduction

0:46

Tissue Preparation

1:18

Cryosection Acquisition

3:24

Matrix Preparation

4:13

Matrix Deposition

6:16

Results: Representative Output with Selected m/z Spectra from Mass Spectrometry Acquired at 50-micron Lateral Resolution

7:37

Conclusion

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