JoVE Journal

Biochemistry

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Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

Please note that all translations are AI generated. Click here for the English version.

3.2K Views

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05:50 min

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May 17th, 2021

DOI :

10.3791/62153-v

May 17th, 2021

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Danté Johnson¹, Benjamin Punshon-Smith², Jessica A. Espino¹, Anne Gershenson³, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences, University of Maryland Baltimore, ²Technology Research Center, University of Maryland Baltimore County, ³Department of Biochemistry and Molecular Biology, University of Massachusetts

Podsumowanie

A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP).

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Platform Incubator

Movable XY Stage

Photochemical Oxidation

Protein ligand Interactions

Protein protein Interactions

Automated Six well Plate

Cell Culture Hood

3D Printed Rings

Peristaltic Pump

Hydrogen Peroxide Infusion

Laser Beam Alignment

Integration Software Script

Cell Dynamics Study

Rozdziały w tym wideo

0:04

Introduction

0:45

Setting Up the Platform Incubator for IC-FPOP

2:08

IC-FPOP in the Platform Incubator

3:41

Results: Analysis of Cell Transfection Efficiency, Proteins Modified in Single Cell Flow-System and PIXY, and Localization of IC-FPOP Modifications

5:22

Conclusion

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