Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

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6.0K Views

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07:35 min

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July 10th, 2021

DOI :

10.3791/62219-v

July 10th, 2021

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Camila Gachet-Castro*¹, Lays Adrianne Mendonça Trajano-Silva*¹, Munira Muhammad Abdel Baqui¹

¹Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil

Podsumowanie

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Double Labeling Immunofluorescence

Antibodies

Host pathogen Interactions

Polyclonal Antibodies

Monoclonal Antibodies

Trypanosoma Cruzi

LLC MK2 Cells

Immunofluorescence Assay

Rozdziały w tym wideo

0:04

Introduction

1:21

Parasite Cultures

2:00

Control Immunofluorescence Protocol

3:23

Double Labeling Immunofluorescence ProtocolUsing Monoclonal and Polyclonal Antibodies Raised in the Same Host

4:18

Triple Labeling Immunofluorescence Protocol with the Addition of the Third Polyclonal Antibody from Different Host Species

5:16

Confocal Imaging Acquisition

5:39

Results: Host-pathogen Interactions Studies Using Double Labeling Immunofluorescence

6:51

Conclusion

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