Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Please note that all translations are AI generated. Click here for the English version.

4.2K Views

•

06:00 min

•

September 20th, 2021

DOI :

10.3791/62901-v

September 20th, 2021

•

Janna Leiz*¹^,², Christian Hinze*¹^,², Anastasiya Boltengagen³, Caroline Braeuning⁴, Christine Kocks³, Nikolaus Rajewsky³, Kai M. Schmidt-Ott¹^,²

¹Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, ²Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ³Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ⁴Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)

Podsumowanie

Przeglądaj więcej filmów

Nuclei Isolation

Adult Mouse Kidney

Single nucleus RNA Sequencing

Gene Expression Data

Cortex Dissection

RNA Stabilization Solution

Nuclei Lysis Buffer 1

Tissue Homogenization

Microdissection

RNA Degradation

Protocol Presentation

Schmidt Ott Lab

Collection Tube

EZ Nuclei Lysis Buffer

Rozdziały w tym wideo

0:04

Introduction

0:41

Tissue Preparation

1:41

Tissue Homogenization and Cell Lysis

3:11

Nuclei Purification

4:40

Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney

5:30

Conclusion

Powiązane Filmy

10:58

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

22.0K Views

07:42

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

82.2K Views

08:06

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

18.0K Views

08:14

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

15.1K Views

10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

30.4K Views

06:28

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

5.6K Views

08:51

Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining

2.2K Views

07:12

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

3.7K Views

05:59

Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing

532 Views