Methods Article

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

4.2K Views

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06:00 min

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September 20th, 2021

DOI :

10.3791/62901-v

September 20th, 2021

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Janna Leiz*¹^,², Christian Hinze*¹^,², Anastasiya Boltengagen³, Caroline Braeuning⁴, Christine Kocks³, Nikolaus Rajewsky³, Kai M. Schmidt-Ott¹^,²

¹Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, ²Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ³Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ⁴Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)

Summary

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Nuclei Isolation

Adult Mouse Kidney

Single nucleus RNA Sequencing

Gene Expression Data

Cortex Dissection

RNA Stabilization Solution

Nuclei Lysis Buffer 1

Tissue Homogenization

Microdissection

RNA Degradation

Protocol Presentation

Schmidt Ott Lab

Collection Tube

EZ Nuclei Lysis Buffer

Chapters in this video

0:04

Introduction

0:41

Tissue Preparation

1:41

Tissue Homogenization and Cell Lysis

3:11

Nuclei Purification

4:40

Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney

5:30

Conclusion

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