A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

3.9K Views

07:59 min

September 10th, 2021

DOI :

10.3791/63012-v

September 10th, 2021


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Streptomyces

Rozdziały w tym wideo

0:05

Introduction

0:58

Preparation of Streptomyces venezuelae ATCC 10712 Cells

3:56

Cell Lysis by Sonication

4:47

Cell Extract Clarification and Run-off Reaction

5:14

Set-up a Standard Streptomyces venezuelae Transcription-Translation (TX-TL) Reaction

6:21

Results: Streptomyces Transcription Translation Toolkit with the pTU1-A-SP44-mScarlet-I Plasmid

7:18

Conclusion

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