Preparation of Streptomyces venezuelae ATCC 10712 Cells
3:56
Cell Lysis by Sonication
4:47
Cell Extract Clarification and Run-off Reaction
5:14
Set-up a Standard Streptomyces venezuelae Transcription-Translation (TX-TL) Reaction
6:21
Results: Streptomyces Transcription Translation Toolkit with the pTU1-A-SP44-mScarlet-I Plasmid
7:18
Conclusion
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Our protocol provides a simplified workflow for Streptomyces venezuelae cell-free transcription-translation. Our system harnesses the metabolic enzymes and pathways from this non-model organism. The main advantages are the heterologous expression
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This protocol details an enhanced method for synthesizing high yields of recombinant proteins from a Streptomyces venezuelae cell-free transcription-translation (TX-TL) system.