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A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

3.9K Views

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07:59 min

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September 10th, 2021

DOI :

10.3791/63012-v

September 10th, 2021

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3,858 Views

Ming Toh¹^,²^,³^,⁴, Kameshwari Chengan⁵, Tanith Hanson⁵, Paul S. Freemont¹^,²^,³^,⁴^,⁶^,⁷, Simon J. Moore⁵

¹Centre for Synthetic Biology and Innovation, South Kensington Campus, ²Department of Medicine, South Kensington Campus, ³Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, ⁴Sir Alexander Fleming Building, South Kensington Campus, ⁵School of Biosciences, Division of Natural Sciences, University of Kent, ⁶UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, ⁷UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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要約

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Streptomyces Venezuelae

Cell free Transcription translation

High GC Content

Metabolic Enzymes

Biosynthetic Pathways

Heterologous Expression

Natural Product Applications

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