Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

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2.3K Views

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09:22 min

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May 31st, 2022

DOI :

10.3791/63322-v

May 31st, 2022

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Ashley Phetsanthad*¹, Nhu Q. Vu*¹, Lingjun Li¹^,²

¹Department of Chemistry, University of Wisconsin-Madison, ²School of Pharmacy, University of Wisconsin-Madison

Podsumowanie

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Neuropeptide Biomarkers

Peptide Extraction

Acidified Methanol

Ultrasonic Homogenizer

Rozdziały w tym wideo

0:04

Introduction

0:49

LC-ESI-MS Analysis of Neuropeptides

2:45

MALDI-MS Spotting Analysis of Neuropeptides

4:13

MALDI-MS Imaging Analysis of Neuropeptides

5:36

Discovering Novel Putative Neuropeptides Using De Novo Sequencing and Transcriptome Mining for Predicted Neuropeptide Sequences

6:57

Results: De Novo Sequencing and MALDI-MS Imaging of Neuropeptides

8:41

Conclusion

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