JoVE Logo

Zaloguj się

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

3.2K Views

09:20 min

April 11th, 2022

DOI :

10.3791/63720-v

April 11th, 2022


Przeglądaj więcej filmów

RNA Fragments

Rozdziały w tym wideo

0:09

Introduction

1:05

Concentration Determination of RNA Solution

3:15

Hydrolysis of Oligonucleotides of RNA by Xrn-1

5:31

Desalting of RNA Solution, MALDI-TOF Plate Spotting

7:41

Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry

8:32

Conclusion

Powiązane Filmy

article

07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

80.4K Views

article

10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

8.0K Views

article

13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

13.4K Views

article

11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

18.9K Views

article

05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

1.8K Views

article

09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

8.2K Views

article

06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

17.7K Views

article

09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

3.0K Views

article

10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

3.9K Views

article

09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

11.9K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone