Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

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3.2K Views

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09:20 min

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April 11th, 2022

DOI :

10.3791/63720-v

April 11th, 2022

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Shawn W. Schowe¹, Conner J. Langeberg², Erich G. Chapman², Kitty Brown³, Marino J. E. Resendiz¹

¹Department of Chemistry, University of Colorado, Denver, ²Department of Chemistry & Biochemistry, University of Denver, ³Analytical Resources Core Bioanalysis & Omics, Colorado State University

Podsumowanie

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RNA Fragments

Enzymatic Degradation

MALDI TOF

Mass Spectrometry

Characterization

In Vitro Applications

Biochemical Transformations

Chemical Modifications

Biopolymer Interactions

Detection Limit

UV Vis Spectrophotometer

Peltier Temperature Controller

Scanning Parameters

Data Collection

Multiple Cell Peltier

Rozdziały w tym wideo

0:09

Introduction

1:05

Concentration Determination of RNA Solution

3:15

Hydrolysis of Oligonucleotides of RNA by Xrn-1

5:31

Desalting of RNA Solution, MALDI-TOF Plate Spotting

7:41

Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry

8:32

Conclusion

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