Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

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2.9K Views

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09:47 min

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August 12th, 2022

DOI :

10.3791/63918-v

August 12th, 2022

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Brikena Gusek¹, Robert Folk¹, Tala Curry², Mitra Esfandiarei¹

¹Department of Biomedical Sciences, College of Graduate Studies, Midwestern University, ²Department of Child Health, College of Medicine-Phoenix, University of Arizona, Phoenix

Podsumowanie

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Endothelium dependent Vasorelaxation

Mouse Thoracic Aorta

Tensometric Myography

Isometric Measurement

Blood Vessel Reactivity

HEPES PSS Solution

High potassium Solution

Silicone Elastomer coated Dish

Segment Dissection

Research Protocol

Rozdziały w tym wideo

0:05

Introduction

0:56

Myograph Unit Preparation

2:03

Isolation and Mounting of the Aortic Segments onto the Myograph Chamber

3:24

Measurement of Endothelium-Dependent Vasorelaxation in Aortic Rings

5:18

Effects of General Inhibitors of NO Production on Endothelium-Mediated Vasorelaxation

6:25

Contribution of the Endothelial Layer to Aortic Vasorelaxation

8:00

Results: Mechanisms Underlying Endothelial-Dependent Vasorelaxation Using Tensometric Small Volume Chamber Myography in a Murine Model

9:06

Conclusion

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