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FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

5.9K Views

11:25 min

June 8th, 2022

DOI :

10.3791/63983-v

June 8th, 2022


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FACS Isolation

Rozdziały w tym wideo

0:04

Introduction

1:04

Hind Limb Muscle Harvesting

3:11

Mechanical and Enzymatic Muscle Digestion

5:04

Generation of Mononucleated Cells

7:03

Antibody Staining for Flow Cytometry

8:49

Results: FACS-Assisted Simultaneous Isolation and Culture of FAPs and MuSCs from Mouse Limb Muscles

10:37

Conclusion

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