Results: FACS-Assisted Simultaneous Isolation and Culture of FAPs and MuSCs from Mouse Limb Muscles
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Transcript
This protocol describes a method for the simultaneous isolation of fibro adipogenic, progenitors, and muscle stem cells using fluorescence activators of sorting. Pure populations of these cells are a prerequisite for understanding their biological
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The precise identification of fibro-adipogenic progenitor cells (FAPs) and muscle stem cells (MuSCs) is critical to studying their biological function in physiological and pathological conditions. This protocol provides guidelines for the isolation, purification, and culture of FAPs and MuSCs from adult mouse muscles.