Methods Article

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

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1.1K Views

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06:36 min

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November 30th, 2022

DOI :

10.3791/64402-v

November 30th, 2022

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Limor Man¹, Nicole Lustgarten Guahmich¹, Eleni Kallinos¹, Laury Arazi¹, Zev Rosenwaks¹^,², Daylon James¹^,²

¹Center for Reproductive Medicine and Infertility, Weill Cornell Medical College, ²Tri-Institutional Stem Cell Derivation Laboratory, Weill Cornell Medical College

Podsumowanie

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Cell Lineage Extraction

Fluorophore Conjugated Antibodies

Cell Surface Markers

Enzymatic Cell Detachment Medium

Rozdziały w tym wideo

0:05

Introduction

0:45

Processing the Ovarian Tissue and Isolation of Antral Follicles

1:50

Isolation of Follicle Resident Cells

3:01

Fluorescence-Activated Cell Sorting

3:36

Processing of the Ovarian Cortical Tissue and Ovarian Tissue Slow Freezing

4:24

Results: Identification and Purification of Ovarian Cells from Antral Follicles

5:45

Conclusion

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