Processing the Ovarian Tissue and Isolation of Antral Follicles
1:50
Isolation of Follicle Resident Cells
3:01
Fluorescence-Activated Cell Sorting
3:36
Processing of the Ovarian Cortical Tissue and Ovarian Tissue Slow Freezing
4:24
Results: Identification and Purification of Ovarian Cells from Antral Follicles
5:45
Conclusion
Transkript
The protocol demonstrated here enables the labeling and isolation of specific cell lineages that are present in ovarian follicles beginning at early antral stages, thereby enabling the high resolution analysis and culture. This highly precise appr
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Here, we present protocols for the identification and purification of ovarian cells from antral follicles. We elaborate on methods for processing whole ovaries for the cryopreservation of cortical strips while also harvesting intact antral follicles that are treated enzymatically to liberate multiple follicle resident cell types, including granulosa, theca, endothelial, hematopoietic, and stromal cells.