High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
2.6K Views
•
10:47 min
•
May 5th, 2023
DOI :
May 5th, 2023
•Chapters in this video
0:00
Introduction
1:21
Preparation of siRNA Mixture for Mitochondrial DNA Synthesis
2:00
Cell Transfection and 5‐Bromo‐2’‐Deoxyuridine (BrdU) Incorporation in HeLa Cells
3:14
5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells
4:51
HeLa Cell Fixation with Hoechst 33342 and Blocking
5:51
Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution
7:49
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells
Related Videos
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
12.9K Views
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
8.5K Views
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
8.0K Views
A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
8.0K Views
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
7.1K Views
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
8.6K Views
Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
7.2K Views
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
9.0K Views
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
4.1K Views
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
3.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved