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Field Collection and Laboratory Maintenance of Canopy-Forming Giant Kelp to Facilitate Restoration

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1.5K Views

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14:44 min

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June 7th, 2024

DOI :

10.3791/66092-v

June 7th, 2024

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Phoebe D. Dawkins¹, Andrea Paz-Lacavex², Evan A. Fiorenza¹, Makena A. Rush¹, Rodrigo Beas-Luna³, Julio Lorda⁴, Luis Malpica-Cruz⁵, Jose M. Sandoval-Gil⁵, Tristin A. McHugh⁶, Min K. Han¹, Matthew E. S. Bracken¹, Joleah B. Lamb¹

¹Department of Ecology and Evolutionary Biology, University of California, Irvine, ²Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, ³Facultad de Ciencias Marinas, Universidad Autónoma de Baja California, ⁴Facultad de Ciencias, Universidad Autónoma de Baja California, ⁵Instituto de Investigaciones Oceanológicas, Universidad Autónoma de Baja California, ⁶The Nature Conservancy

Podsumowanie

This protocol describes the field collection and regular laboratory maintenance of substrates seeded with canopy-forming giant kelp for use in restoration trials to address the success and limitations of the 'green gravel' technique in field settings.

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Macrocystis Pyrifera

Kelp Restoration

Field Collection

Laboratory Maintenance

Green Gravel Technique

Culturing Protocol

Environmental Stressors

Temperature Control

Full Spectrum Light

Incubator Systems

PAR Quantum Meter

Bacterial Contamination

Substrate Rugosity

Gravel Preparation

Filter sterilized Seawater

Rozdziały w tym wideo

0:05

Introduction

0:35

Preparation of Facilities and Materials

2:27

Preparation of Growth Media

3:52

Field Collection

5:38

Sporulation

7:50

Inoculation

8:35

Rearing Conditions

9:05

Monitoring

10:58

Maintenance

12:02

Deployment

12:45

Results

14:21

Conclusion

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