Nuclear Transfer into Mouse Oocytes

0:17

Preparation of dishes

0:18

Dish for oocyte incubation KSOM

1:08

Dish for oocyte isolation HCZB and HCZB/hyaluronidase

2:23

Dish for enucleation and nuclear transfer HCZB/cytocholazin

3:30

Preparation of pipettes

3:31

Holding pipette

4:53

Injection pipette

6:30

Mouth pipette for oocyte isolation

7:07

Oocyte isolation

9:43

Nuclear transfer

9:58

Enucleation

10:31

Collection of donor cells

11:05

Transfer

11:50

Discussion