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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

14.2K Views

13:06 min

December 15th, 2012

December 15th, 2012

14,158 Views

0:05

Title

1:38

Sexual Cycling

6:13

Flow Cytometry

9:26

Genotyping

10:28

Results: Yeast Strains Carrying Multiple Gene Deletions as Generated by the Green Monster Process

12:47

Conclusion

The Green Monster method enables the rapid assembly of multiple deletions marked with a reporter gene encoding green fluorescent protein. This method is based on driving yeast strains through repeated cycles of sexual assortment of deletions and fluorescence-based enrichment of cells carrying more deletions.

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