A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
August 31st, 2013
•We developed a quantitative DNA-binding, ELISA-based assay to measure transcription factor interactions with DNA. High specificity for the RUNX2 protein was achieved with a consensus DNA-recognition oligonucleotide and specific monoclonal antibody. Colorimetric detection with an enzyme-coupled antibody substrate reaction was monitored in real time.
Tags
Vídeos Relacionados
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
In vitro Reconstitution of the Active T. castaneum Telomerase
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados