Results: Analysis of Brain Mitochondrial Morphology, Distribution, Abundance and Ultrastructure Using Serial Block-Face Scanning Electron Microscopy
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The overall goal of the serial block-face scanning electron microscopy technique is to analyze mitochondrial morphology, structure, distribution, volume, and number in a defined region of the mouse brain. This method can help answer many key quest
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Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.