The overall goal of this procedure is to detect differentiated interrenal steroidogenic cells in the developing zebrafish through a simple whole-mount enzymatic activity assay. This staining procedure can help answer key questions regarding the mo
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The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.