Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
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Conclusion
TRANSCRIÇÃO
The overall goal of this procedure is to measure the insulin stimulated glucose uptake in human primary muscle cells. This method can help answer the key questions in the muscle metabolism field, such as muscle cell insulin sensitivity. The main a
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In this method, human primary muscle cells are cultured in vitro to obtain differentiated myotubes and glucose uptake rates are measured. We provide a detailed protocol to quantify rates in basal and insulin-stimulated states using radiolabeled [3H] 2-deoxy-D-Glucose.