January 22nd, 2019
•This paper uses a flow-cytometry-based assay to screen libraries of chemical inhibitors for the identification of inhibitors and their targets that influence T-cell receptor signaling. The methods described here can also be expanded for high-throughput screenings.
Tags
Vídeos Relacionados
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Real-time Live Imaging of T-cell Signaling Complex Formation
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados