Microinjection and Heat Shock of Zebrafish Embryos
2:06
Screening and Mounting Embryos
3:59
Time-lapse Confocal Imaging of the Developing Zebrafish Hindbrain
5:17
Results: Brainbow Labels Clonally Related Clusters of Dividing Cells in the Developing Zebrafish Hindbrain
6:55
Conclusion
Transcrição
This method can help answer fundamental questions about the dynamics of neural progenitor cells and their progeny which underlie vertebrate brain development. The main advantage of this technique is the ability to directly visualize multiple clust
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In vivo imaging is a powerful tool that can be used to investigate the cellular mechanisms underlying nervous system development. Here we describe a technique for using time-lapse confocal microscopy to visualize large numbers of multicolor Brainbow-labeled cells in real time within the developing zebrafish nervous system.