Results: Visualization and Quantification of Membrane Ruffle Formation
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Conclusion
TRANSCRIÇÃO
The present SEM imaging protocol provides a tool to visualize and quantify membrane ruffle formation, circular protrusions, and macropinocytic cups on the cell surface in vitro. SEM provides high-resolution images of the cell surface that can be u
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Macropinocytosis is a highly conserved endocytic process initiated by the formation of F-actin-rich sheet-like membrane projections, also known as membrane ruffles. Increased rate of macropinocytotic solute internalization has been implicated in various pathological conditions. This protocol presents a method to quantify membrane ruffle formation in vitro using scanning electron microscopy.