Cell-Free Protein Synthesis and Quantification of the Reporter Protein, Super Folder Green Fluorescent Protein (sfGFP)
4:46
Results: Comparison Between Cell Extracts Obtained from Cell-Free Autoinduction Media Grown to Optical Densities of 2.5 and 10
5:27
Conclusion
Transcrição
The cell-free autoinduction method reduces researcher oversight and time needed to produce functional cell extracts. This simplifies previous in vitro protein synthesis protocols that were more time-consuming and intensive. This technique is less
Faça login ou inicie seu teste gratuito para acessar este conteúdo
This work describes the preparation of cell extract from Escherichia coli (E. coli) followed by cell-free protein synthesis (CFPS) reactions in under 24 hours. Explanation of the cell-free autoinduction (CFAI) protocol details improvements made to reduce researcher oversight and increase quantities of cell extract obtained.