This protocol describes a high throughput colorimetric assay in the three lifecycle stages of Trypanosoma cruzi to identify new drug candidates for Chagas disease. This can be used to identify trypanocidal compounds in an easy, fast, and reproduci
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We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.