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High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

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10:08 min

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July 6th, 2022

July 6th, 2022

•
3,965 Views

0:04

Introduction

0:52

Collecting Leaf Samples from Field

2:11

Preparing Samples for Analysis

4:07

Measuring of Non-Photochemical Quenching Using Chlorophyll Fluorescence Imager

8:45

Results: High-Throughput Method for Measuring the Relaxation of NPQ in Field-Grown Soybeans

9:27

Conclusion

Transcrição

This method can provide an insight into the genetic variation in non-photochemical quenching available within genetic diversity panels or breeding populations. The main advantage of this technique is the potential to screen a large number of genot

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The protocol introduces a high-throughput method for measuring the relaxation of non-photochemical quenching by pulse amplitude modulated chlorophyll fluorometry. The method is applied to field-grown Glycine max and can be adapted to other species to screen for genetic diversity or breeding populations.

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