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JoVE Journal

Bioengineering

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Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

3.2K Views

08:19 min

June 23rd, 2022

June 23rd, 2022

3,174 Views

0:05

Introduction

0:48

Device Fabrication

4:51

Device Inoculation

6:01

Results: Visualization of Bacterial-Fungal and Fungal-Fungal Interactions

7:38

Conclusion

Transcrição

Using the presented microfluidic devices, we can examine dynamic interactions between fungi and other microbes with precise spacial and environmental control, as well as high-resolution imaging at the single cell level. Importantly, fungal growth

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Owing to the opacity of soil, interactions between its constituent microbes cannot easily be visualised with cellular resolution. Here, two microfluidic tools are presented, which offer new opportunities for investigating fungal-microbial interactions. The devices are versatile and simple to use, enabling high spatiotemporal control and high-resolution imaging at the cellular level.

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