JoVE Logo
Centro de Recursos para Docentes

Entrar

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

DOI :

10.3791/64868-v

September 22nd, 2023

September 22nd, 2023

881 Views

1Department of Microbiology, University of Pennsylvania, 2Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine, University of Pennsylvania, 3Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, 4Corporal Michael J. Crescenz VA Medical Center

The nasal epithelium is the primary barrier site encountered by all respiratory pathogens. Here, we outline methods to use primary nasal epithelial cells grown as air-liquid interface (ALI) cultures to characterize human coronavirus-host interactions in a physiologically relevant system.

Tags

Infection

-- Visualizações

Vídeos Relacionados

article

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

article

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

article

Following Cell-fate in E. coli After Infection by Phage Lambda

article

An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells

article

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

article

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

article

Biomimetic Materials to Characterize Bacteria-host Interactions

article

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

article

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue

article

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

JoVE Logo

Privacidade

Termos de uso

Políticas

Pesquisa

Educação

SOBRE A JoVE

Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados