<a href="https://www.jove.com/video/3527">Хитозан основе, лазерная активированный тонкопленочных хирургических клей », SurgiLux&quot;: подготовка и демонстрация.</a> Л. Джон Р. Фостер, Элизабет Karsten Bio / Полимерные Research Group, Университетом Нового Южного Уэльса Изготовление новых, гибких тонкопленочных хирургического клея от FDA утвержденных ингредиентов, хитозан и индоцианин зеленый описано. Приклеивание этого клея на коллагеновой ткани через простой процесс активации с маломощным инфракрасным лазером показала. <a href="https://www.jove.com/video/4158">Изготовление и применение бенгальским розовым-хитозан Фильмы в лазерной восстановления тканей</a> Антонио Lauto 1, Маркус Stoodley 2, Мэтью Бартон 1, Джон Морли 1, David A. Mahns 1, Леонардо Лонго 3, Damia Mawad <sup&gt; 1 1 Биоэлектроника и неврологии (BENS) исследовательской группой Университета Западного Сиднея, Новый Южный Уэльс Австралии, 2 австралийская школа повышения квалификации врачей, Macquarie University, Южный Уэльс, Австралия, 3 школы медицины Университета Сиены, Италия Швы, как правило, необходимы для восстановления тканей во время хирургических процедур. Однако их применение может быть проблематичным, поскольку они являются инвазивными и может привести к повреждению тканей. Изготовление и применение методов романа клей ткани здесь сообщается. Этот клей пленки лазерным активированы и не требуют использования швов. <a href="https://www.jove.com/video/4193">Гальванотаксис пробирного анализа нейронных клеток-предшественников Кинетика миграции извне постоянного тока электрическое поле</a> Робарт Babona-Pilipos 1, Милош Р. Поповича 2, Синди М. Morshead 3 1Институт биоматериалов и биомедицинской инженерии, Университет Торонто, 2 Lyndhurst Centre, Торонто реабилитации института, 3 отделение хирургии, Университет Торонто В этом протоколе мы покажем, как для создания пользовательских камер, что позволяет применять постоянный ток электрического поля для включения покадровой визуализации мозга взрослого человека, полученных нейронных перемещение клеток-предшественников во время гальванотаксиса. <a href="https://www.jove.com/video/4214">Сотовые конкретного анализа листьев Arabidopsis с помощью флуоресцентной активирован сортировки клеток</a> Jesper Т. Гронлунд 1, Элисон Eyres 1, Санджив Кумар 1, Vicky Бьюкенен-Волластон 1, 2, Мириам Л. Гиффорд 1, 2 1 Школа естественных наук, Университет Уорвика, 2 Warwick системной биологии, Университет Уорвика Способ получения протопластов листьев Arabidopsis, которые совместимы с флуоресценции активирован сортировки клеток (FACS), что позволяет для исследования конкретных клеточных популяций. Этот метод совместим с любыми Arabidopsis линии, которая выражает GFP в подмножество клеток. <a href="https://www.jove.com/video/4227">Визуализация бактериальный токсин индуцированные ответы с помощью флуоресцентной микроскопии Онлайн сотовых</a> Петр Алексеевич Keyel 1, Мишель Е. Heid 1, Simon C. Watkins 2, Russell D. Salter 1 1 Кафедра иммунологии, Университет Питтсбурга школа медицины, 2 отдела клеточной биологии и физиологии Университета Питтсбурга школа медицины Методы очистки холестерина обязательного токсина стрептолизин O из рекомбинантной E. палочки и визуализации токсина обязательным жить эукариотической клетки описыватьD. Локализованные доставки токсин вызывает быстрые и сложные изменения в целевые клетки раскрывая новые аспекты токсина биологии. <a href="https://www.jove.com/video/4247">Высокая пропускная способность последовательного ELISA для проверки биомаркеров острого трансплантат против хозяина</a> Брайан Fiema *, Эндрю С. Харрис *, Орели Гомес, Praechompoo Pongtornpipat, Келли Lamiman, Марк Т. Вандер Lugt, Софи Paczesny Детская крови и пересадке мозга программе, Мичиганский университет * Эти авторы способствовали в равной степени Высокая пропускная проверки нескольких биомаркеров кандидат может быть выполнена последовательная ELISA для того, чтобы свести к минимуму циклов замораживания / оттаивания и использования драгоценных образцов плазмы. Здесь мы покажем, как последовательно выполнять ИФА для шести различных подтверждено биомаркеров плазмы 1-3 трансплантат против хозяина (РТПХ) 4 на той же плОУРА образца. <a href="https://www.jove.com/video/4448">Анализ изображений нейрона к Glia Взаимодействие в Микрофлюидных Платформа культуры (MCP) на основе нейронных аксонов и глии Co-культуре системы</a> Харуки Higashimori 1, Yongjie Ян 1, 2 1 Кафедра неврологии, Университет Тафтса, 2 Neuroscience программы, Тафтс Sackler школа Высшее медико-биологических наук Данное исследование описывает процедуры создания новых нейронных аксонов и (ASTRO) глии совместного культивирования платформы. В этой совместной системе культуры, манипуляции прямого взаимодействия между одним аксона (и единственной глиальных клеток) становится возможным, позволяя механистического анализа взаимного нейрона к глиальных сигнализации. <a href="https://www.jove.com/video/50129">Фотоэмиссии с угловым разрешением спектроскопии при сверхнизких температурах</a> Сергей Владимирович Borisenko 1, Владимир Б. Zabolotnyy 1, А. В. Kordyuk 1, 2, Данил В. Evtushinsky 1, Тимур К. Ким 1, 3, Emanuela Carleschi 4, Брайан Дойл П. 4, Rosalba Фиттипальди 5, Марио Cuoco 5, Антонио Vecchione 5, Хельмут Бергер 6 1 Институт твердого Государственный научно-исследовательский, IFW-Dresden, 2 Институт физики металлов Национальной академии наук Украины, 3 Diamond Light Source LTD, 4, физический факультет Университета Йоханнесбурга, 5 CNR-SPIN, и Dipartimento ди Fisica &quot;ER Caianiello &quot;, Università ди Салерно, 6 Институт физики сложный вопрос, Федеральной политехнической школе Лозанны Основная цель этого метода состоит в определении низких энергий электронной структуры твердых тел при сверхнизких температурах с помощью фотоэмиссии с угловым разрешением спектроскопияКопировать с синхротронного излучения. <a href="https://www.jove.com/video/261">Изготовление микрофлюидных устройство для изолированность нейрона Сома и Аксоны</a> Джозеф Харрис 1, Hyuna Ли 1, Behrad Вахиди 1, Кристина Ту 2, Дэвид Cribbs 3, Ноо Ли Чон 1, Карл Cotman 3 1 Кафедра биомедицинской инженерии, Университет Калифорнии в Ирвине, 2 Stem Cell Research Center, Калифорнийский университет, Ирвайн, 3 института старения мозга и слабоумие, Калифорнийский университет, Ирвайн В этом видео мы продемонстрируем технику мягкой литографии с полидиметилсилоксана (PDMS), который мы используем, чтобы farbricate микрофлюидных устройство для культивирования нейронов. <a href="https://www.jove.com/video/305">Подготовка E18 корковых нейронов крысы для раздробленности в микрофон</a>rofluidic устройств Джозеф Харрис 1, Hyuna Ли 1, Кристина Tu Tu 2, Дэвид Cribbs 3, Карл Cotman 3, Ноо Ли Чон 1 1 Кафедра биомедицинской инженерии, Университет Калифорнии в Ирвине, 2 Stem Cell Research Center, Калифорнийский университет, Ирвайн, 3 института старения мозга и слабоумие, Калифорнийский университет, Ирвайн В этом видео мы покажем, подготовка E18 корковых нейронов крысы. <a href="https://www.jove.com/video/410">Non-плазменной Склеивание PDMS для недорогих Изготовление микрожидкостных устройств</a> Джозеф Харрис 1, Hyuna Ли 1, Behrad Вахиди 1, Кристина Ту 2, Дэвид Cribbs 3, Карл Cotman 3, Ноо Ли Чон 1 1 Кафедра медико-биологических Engineerinг, Калифорнийский университет, Ирвайн, 2 Stem Cell Research Center, Калифорнийский университет, Ирвайн, 3 института старения мозга и слабоумие, Калифорнийский университет, Ирвайн В этом видео мы покажем, как с помощью нейронных микрофлюидных устройство без связи плазме.

Нет конфликта интересов объявлены.

october 2012: this month in jove

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE). Gr&oslash;nlund et al. demonstrate how to isolate and analyze specific cell types in plant leaves expressing green fluorescent protein (GFP) using fluorescence-activated cell sorting (FACS). This method overcomes the interfering fluorescence of the chlorophyll found in leaves, and distinguishes GFP-expressing protoplasts from non-GFP protoplasts. In JoVE Neuroscience, Babona-Pilipos et al. demonstrate how to construct a chamber for measuring galvanotaxis, or cell migration within an electric field. Through time-lapse imaging and image analysis, the authors can study the migratory behavior of neural precursor cells in an electric field, which may lead to the use of electrical stimulation to direct neural precursors to sites of injury or disease. Articles involving microfluidic platforms have a significant publication history in JoVE. Harris et al. have released three articles, which involve a microfluidic device for separating axons from neuronal cell bodies. This month in JoVE Neuroscience, Higashimori et al. use this microfluidic platform to investigate the interactions between neuronal axons and glial cells, which are critical for the physiological function of the nervous system. In JoVE Bioengineering, two research groups demonstrate the novel bioadhesive properties of chitosan-a polymer derived from chitin, which is found in fungal cell walls or in the exoskeletons of crustaceans and insects. Chitosan is used in many industrial and agricultural applications, and bioengineers are also finding uses for it in surgical applications. Foster et al. have developed a laser-activated surgical film called Surgilux, which combines chitosan with indocyanine green ICG), a photosensitive dye. This surgical film binds strongly to tissue, such as muscle, after laser irradiation. Lauto et al. developed a surgical film that combines chitosan with the photoactive dye, rose Bengal. This novel film also binds strongly to tissues, such as intestine, after it is irradiated. These adhesive films are biocompatible and may potentially be used in various surgical procedures in place of sutures. In JoVE Clinical and Translational Medicine, Fiema et al. demonstrate a high-throughput technique for validating biomarkers in graft vs. host disease, a common and life-threatening complication of cell or tissue transplantation. The authors use commercially available ELISAs to analyze multiple proteins in sequential fashion. In JoVE Immunology and Infection, Keyel et al. demonstrate how to measure the real-time kinetics of immune cell responses to bacterial toxins using high-speed live cell microscopy. This method can be used to show how immune cells respond to bacterial toxins. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response. In JoVE Applied Physics, Borisenko et al. determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy in a synchotron radiation facility. By combining recent advances in synchotron radiation, surface science, and cryogenics, this method can gain a precise picture of the energy and momentum of electrons inside a solid, and address key questions in the field of condensed matter physics. This preview summarizes just a few of the notable video-articles available in the October 2012 issue of JoVE. For additional videos, please visit <a href="http://www.jove.com">www.jove.com</a>.

Watch this Scientific Journal Video about October 2012: This Month in JoVE at JoVE.com

October 2012: This Month in JoVE

Вот некоторые основные моменты из октябре 2012 вопрос о журнале Визуализированные Эксперименты (Юпитера).

Октябрь 2012: В этом месяце в Юпитер

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE).  Gr&oslash;nlund et al. demonstrate how to isolate and ...

Research

JoVE Journal

Biology

10.2K Views.  Massachusetts Eye and Ear. Вот некоторые основные моменты из октябре 2012 вопрос о журнале Визуализированные Эксперименты (Юпитера).

Video: October 2012: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Август 2012: В этом месяце в Юпитер

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: год в обзоре

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...