<a href="https://www.jove.com/video/3527">A Chitosan Based, Laser Activated Thin Film Surgical Adhesive &quot;SurgiLux &#39;: Vorbereitung und Demonstration.</a> L. John R. Foster, Elizabeth Karsten Bio / Polymer Research Group, University of New South Wales Die Herstellung einer erfindungsgemäßen, flexiblen Dünnfilm chirurgischen Klebstoff aus FDA zugelassenen Inhaltsstoffen, Chitosan und Indocyaningrün beschrieben. Bonden dieser Klebstoff auf Kollagengewebe durch eine einfache Aktivierung mit einer Low-Power-Infrarotlaser nachgewiesen wird. <a href="https://www.jove.com/video/4158">Herstellung und Anwendung von Rose Bengal-Chitosan-Films in Laser Tissue Repair</a> Antonio lauto 1, Marcus Stoodley 2, Matthew Barton 1, John W. Morley 1, David A. Mahns 1, Leonardo Longo 3, Damia Mawad <sup&gt; 1 1 Bioelektronik und Neuroscience (BENS) Forschungsgruppe der University of Western Sydney, NSW Australien, 2 Australian School of Advanced Medicine, Macquarie University, NSW Australien, 3 School of Medicine, University of Siena, Italien Die Fäden werden in der Regel erforderlich, um Gewebe während chirurgischer Eingriffe zu reparieren. Jedoch kann ihre Anwendung als problematisch, da sie invasiv sind und kann Gewebe beschädigen. Die Herstellung und Anwendung Methoden einer neuartigen Gewebekleber werden hier berichtet. Diese Klebefolie wird lasersensitiven aktiviert und erfordert nicht die Verwendung von Nähten. <a href="https://www.jove.com/video/4193">A Galvanotaxis Assay für die Analyse von neuralen Vorläuferzellen Cell Migration Kinetics in einem von außen angelegten Direct Current Electric Field</a> Robart Babona-Pilipos 1, Milos R. Popovic 2, Cindi M. Morshead 3 1Institut für Biomaterialien und Biomedical Engineering, University of Toronto, 2 Lyndhurst Centre, Toronto Rehabilitation Institute, 3 Department of Surgery, University of Toronto In diesem Protokoll zeigen wir, wie Sie benutzerdefinierte Kammern, die die Anwendung eines Gleichstrom elektrisches Feld an Zeitraffer-Aufnahmen von erwachsenen Gehirn abgeleitete neurale Vorläuferzellen Translokation während Galvanotaxis ermöglichen ermöglichen konstruieren. <a href="https://www.jove.com/video/4214">Zelle spezifische Analyse von Arabidopsis Blätter mit fluoreszenzaktivierten Zellsortierung</a> Jesper T. Grønlund 1, Alison Eyres 1, Sanjeev Kumar 1, Vicky Buchanan-Wollaston 1, 2, Miriam L. Gifford 1, 2 1 School of Life Sciences, University of Warwick, 2 Warwick Systems Biology, University of Warwick AVerfahren zur Herstellung von Arabidopsis Blattprotoplasten, die kompatibel mit der fluoreszenzaktivierten Zellsortierung (FACS) sind, so dass für Studien spezifischer Zellpopulationen. Dieses Verfahren ist kompatibel mit jeder Zeile, die Arabidopsis GFP exprimiert in einer Untergruppe von Zellen. <a href="https://www.jove.com/video/4227">Visualisierung der bakteriellen Toxin induzierten Reaktionen mit Live Cell Fluoreszenzmikroskopie</a> Peter A. Keyel 1, Michelle E. Heid 1, Simon C. Watkins 2, Russell D. Salter 1 1 Department of Immunology, University of Pittsburgh School of Medicine, 2 Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine Methoden zur Reinigung des Cholesterin-Bindung Toxin Streptolysin O von rekombinantem E. coli und Visualisierung von Toxin Bindung an eukaryotischen Zellen leben, sind zu beschreibend. Lokalisierte Abgabe von Toxin induziert eine schnelle und komplexe Veränderungen in Zielzellen enthüllen neue Aspekte des Toxins Biologie. <a href="https://www.jove.com/video/4247">High Throughput Sequential ELISA zur Validierung von Biomarkern des akuten Graft-Versus-Host Disease</a> Bryan Fiema *, Andrew C. Harris *, Aurelie Gomez, Praechompoo Pongtornpipat, Kelly Lamiman, Mark T. Vander Lugt, Sophie Paczesny Pediatric Blood and Marrow Transplant Program, University of Michigan * Diese Autoren trugen gleichermaßen Hoher Durchsatz Validierung mehrerer Biomarker-Kandidaten können durch sequentielle ELISA durchgeführt werden, um Einfrieren / Auftauen zu minimieren und die Nutzung von wertvollen Plasmaproben. Hier zeigen wir, wie man nacheinander durchführen ELISAs für sechs verschiedene validierte Plasma-Biomarker 1-3 von Graft-versus-Host-Krankheit (GVHD) 4 auf der gleichen plasma Probe. <a href="https://www.jove.com/video/4448">Imaging Analyse von Neuron zu Glia Interaktionen in Microfluidic Kultur Platform (MCP)-Based Neuronale Axon und Glia Co-Kultur-System</a> Haruki Higashimori 1, Yongjie Yang 1, 2 1 Department of Neuroscience, Tufts University, 2 Neuroscience Program, Tufts Sackler School of Graduate Biomedical Sciences Diese Studie beschreibt die Verfahren für die Einrichtung einer neuartigen neuronalen Axon und (astro) Glia Co-Kultur-Plattform. Auf diese Co-Kultur-System wird Manipulation direkte Wechselwirkung zwischen einem einzelnen Axon (und einzigen Gliazelle) durchführbar ist, so dass mechanistischen Analyse des gegenseitigen Neuron zu glialen Signalisierung. <a href="https://www.jove.com/video/50129">Angle-Photoemissionsspektroskopie Bei extrem niedrigen Temperaturen</a> Sergey V. Borisenko 1, Volodymyr B. Zabolotnyy 1, Alexander A. Kordyuk 1, 2, Danil V. Evtushinsky 1, Timur K. Kim 1, 3, Emanuela Carleschi 4, Bryan P. Doyle 4, Rosalba Fittipaldi 5, Mario Cuoco 5, Antonio Vecchione 5, Helmut Berger 6 1 Institut für Festkörperforschung, IFW-Dresden, 2 Institut für Metallphysik der Nationalen Akademie der Wissenschaften der Ukraine, 3 Diamond Light Source LTD, 4 Department of Physics, University of Johannesburg, 5 CNR-SPIN und Dipartimento di Fisica &quot;ER Caianiello &quot;, Università di Salerno, 6 Institut für Physik komplexer Materie, École Polytechnique Fédérale de Lausanne Das übergeordnete Ziel dieser Methode ist es, die Niedrig-Energie-elektronischen Struktur von Festkörpern bei extrem niedrigen Temperaturen mit Angle-Photoemission Spectrosc bestimmenopy mit Synchrotronstrahlung. <a href="https://www.jove.com/video/261">Herstellung einer mikrofluidischen Vorrichtung für die Kompartimentierung von Neuron Soma und Axone</a> Joseph Harris 1, Hyuna Lee 1, Behrad Vahidi 1, Christina Tu 2, David Cribbs 3, Noo Li Jeon 1, Carl Cotman 3 1 Department of Biomedical Engineering, University of California, Irvine, 2 Stem Cell Research Center, University of California, Irvine, 3 Institut für Hirnforschung Altern und Demenz, University of California, Irvine In diesem Video zeigen wir die Technik der weichen Lithographie mit Polydimethylsiloxan (PDMS), die wir verwenden, um einen mikrofluidischen Vorrichtung zum Kultivieren von Neuronen farbricate. <a href="https://www.jove.com/video/305">Vorbereiten E18 Kortikale Rattenneuronen für Kompartimentierung in einem Mic</a>rofluidic Geräte Joseph Harris 1, Hyuna Lee 1, Christina Tu Tu 2, David Cribbs 3, Carl Cotman 3, Noo Li Jeon 1 1 Department of Biomedical Engineering, University of California, Irvine, 2 Stem Cell Research Center, University of California, Irvine, 3 Institut für Hirnforschung Altern und Demenz, University of California, Irvine In diesem Video zeigen wir die Herstellung von E18 Kortikale Rattenneuronen. <a href="https://www.jove.com/video/410">Nicht-Plasma-Bonding von PDMS für kostengünstige Herstellung von mikrofluidischen Bauteilen</a> Joseph Harris 1, Hyuna Lee 1, Behrad Vahidi 1, Cristina Tu 2, David Cribbs 3, Carl Cotman 3, Noo Li Jeon 1 1 Department of Biomedical Engineering, University of California, Irvine, 2 Stem Cell Research Center, University of California, Irvine, 3 Institut für Hirnforschung Altern und Demenz, University of California, Irvine In diesem Video zeigen wir, wie die Neuronen mikrofluidischen Vorrichtung ohne Plasma Bindung verwenden.

Keine Interessenskonflikte erklärt.

october 2012: this month in jove

Hier sind einige Highlights aus dem Oktober 2012 Ausgabe des Journal of Visualized Experiments (Jupiter). Grønlund et al. Demonstrieren, wie die Isolierung und Aufarbeitung von spezifischen Zelltypen in Pflanzenblätter exprimieren grün fluoreszierendes Protein (GFP) unter Verwendung der Fluoreszenz-aktivierten Zellsortierung (FACS). Dieses Verfahren überwindet die störende Fluoreszenz des Chlorophyll in den Blättern gefunden, und unterscheidet GFP-exprimierenden Protoplasten aus Nicht-GFP Protoplasten. In JoVE Neuroscience, Babona-Pilipos et al. zeigen, wie man eine Kammer zur Messung Galvanotaxis oder Zellmigration in einem elektrischen Feld zu konstruieren. Durch Zeitraffer-Bildgebung und Bildanalyse können die Autoren zu studieren das Zugverhalten von neuralen Vorläuferzellen in einem elektrischen Feld, das die Verwendung der elektrischen Stimulation direkten neuralen Vorläuferzellen zu Websites von Verletzungen oder Krankheit führen kann. Artikel mit microFluidic Plattformen haben einen bedeutenden Publikation Geschichte JoVE. Harris et al  . veröffentlicht drei Artikel, die einen mikrofluidischen Vorrichtung beinhalten zum Trennen Axone von Nervenzellen Körper haben. In diesem Monat in JoVE Neuroscience, Higashimori et al. Mit diesem mikrofluidischen Plattform, um die Interaktionen zwischen neuronalen Axonen und Gliazellen, die entscheidend für die physiologische Funktion des Nervensystems sind, untersuchen. In JoVE Bioengineering zeigen zwei Arbeitsgruppen der erfindungsgemäßen bioadhäsiven Eigenschaften eines Polymers Chitosan aus Chitin ableitet, die in Pilzzellwänden oder in den Exoskelett von Krustentieren und Insekten gefunden wird. Chitosan wird in vielen industriellen und landwirtschaftlichen Anwendungen verwendet, und bioengineers finden auch Anwendungen für sie in chirurgischen Anwendungen. Foster et al. entwickelten ein laseraktiviertenChirurgisches Film genannt Surgilux, die kombiniert mit Chitosan Indocyaningrün ICG), einem lichtempfindlichen Farbstoff enthält. Dieser chirurgische Folie bindet stark an Gewebe wie Muskel, nach der Laserbestrahlung. Lauto et al. entwickelte eine chirurgische Film, Chitosan verbindet sich mit der photoaktive Farbstoff, stieg Bengal. Diese neuartige Folie auch bindet stark an Gewebe wie Darm, nachdem es bestrahlt wird. Diese Klebefolien sind biokompatibel und kann möglicherweise in verschiedenen chirurgischen Verfahren, die von Nähten verwendet werden. In JoVE Clinical and Translational Medicine, Fiema et al. zeigen High-Throughput-Technik für die Validierung von Biomarkern in Graft-versus-host-Krankheit, eine gemeinsame und lebensbedrohliche Komplikation von Zellen oder Geweben Transplantation. Die Autoren verwenden kommerziell erhältlichen ELISAs mehrere Proteine ​​in sequentieller Weise zu analysieren. In JoVE Immunologie und Infektionsbiologie zeigen Keyel et al., Wie die Echtzeit-Kinetik im messenmune Zell-Reaktionen auf bakterielle Toxine mit High-Speed ​​Live Cell Mikroskopie. Diese Methode kann verwendet werden, um zu zeigen, wie Immunzellen bakterielle Toxine reagieren. Kombiniert mit Hochgeschwindigkeits-3D-konfokale Mikroskopie kann diese Technik auch visualisieren die zelluläre Reparatur Reaktion. In JoVE Applied Physics, Borisenko et al. Bestimmung der elektronischen Struktur von komplexen Materialien mit Winkel-Photoemissionsspektroskopie in einem Synchrotron Radiation Facility. Durch die Kombination jüngsten Fortschritte in der Synchrotron-Strahlung, Oberflächenphysik und Kryotechnik, kann diese Methode gewinnen ein genaues Bild von der Energie und Dynamik von Elektronen in einem festen und entscheidende Fragen auf dem Gebiet der Physik der kondensierten Materie. Diese Vorschau fasst nur einige der bemerkenswerten Video-Artikel in der Oktober 2012 Ausgabe von JoVE. Für zusätzliche Videos, besuchen Sie bitte <a href="http://www.jove.com">www.jove.com</a> .

Watch this Scientific Journal Video about October 2012: This Month in JoVE at JoVE.com

October 2012: This Month in JoVE

Hier sind einige Highlights aus der Oktober 2012 Ausgabe des Journal of Visualized Experiments (Jupiter).

Oktober 2012: Diesen Monat in JoVE

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE).  Gr&oslash;nlund et al. demonstrate how to isolate and ...

Research

JoVE Journal

Biology

10.2K Aufrufe.  Massachusetts Eye and Ear. Hier sind einige Highlights aus der Oktober 2012 Ausgabe des Journal of Visualized Experiments (Jupiter).

Serie: October 2012: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

August 2012: Diesen Monat in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Forschung

Biologie

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Das Jahr im Rückblick

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...