In situ TEM of Biological Assemblies in Liquid

Summary

Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.

Abstract

Researchers regularly use Transmission Electron Microscopes (TEMs) to examine biological entities and to assess new materials. Here, we describe an additional application for these instruments- viewing viral assemblies in a liquid environment. This exciting and novel method of visualizing biological structures utilizes a recently developed microfluidic-based specimen holder. Our video article demonstrates how to assemble and use a microfluidic holder to image liquid specimens within a TEM. In particular, we use simian rotavirus double-layered particles (DLPs) as our model system. We also describe steps to coat the surface of the liquid chamber with affinity biofilms that tether DLPs to the viewing window. This permits us to image assemblies in a manner that is suitable for 3D structure determination. Thus, we present a first glimpse of subviral particles in a native liquid environment.

Introduction

A common goal of biologists and engineers is to understand the inner-workings of molecular machines. Transmission Electron Microscopes (TEMs) are ideal instruments to visualize these intricate details at near-atomic resolution^1-2. In order to sustain the high vacuum system of a TEM, biological samples are typically embedded in thin films of vitreous ice³, sugars⁴, heavy metal salts⁵, or some combination thereof⁶. As a result, images of embedded specimens may reveal only limited snapshots of dynamic processes.

Early attempts to maintain biological specimens hydrated in environmental liquid chambers were undertaken by Parsons and colleagues using differential pumping stages. Electron diffraction patterns of unstained catalase crystals were successfully recorded to a resolution of 3 Å in a hydrated state^7-8. In addition, phase-separated lipid domains could be examined in hydrated membranes of human erythrocytes^9-10. However, motion caused by diffusing liquid and its interference with the electron beam, resulted in severe resolution loss and further experiments using biological specimens were not attempted until recently.

Newly developed microfluidic specimen holders have been introduced that utilize semiconductor microchips to form a micro-scaled environmental chamber. These devices can maintain samples in liquid while they are positioned in a TEM column^11-12. This technical breakthrough in TEM imaging has allowed researchers to view, for the first time, progressive events at the molecular level¹³. We refer to this new modality as “in situ molecular microscopy” as experiments can now be performed “inside” the EM column^14-15. The overall goal of this method is to image biological assemblies in liquid in order to observe their dynamic behaviors at nanometer resolution. The rationale behind the developed technique is to record real-time observations and examine new properties of biological machinery in solution. This methodology expands the use of TEMs for broader purposes in cellular and molecular biology^12-16.

In the current video article, we present a comprehensive protocol to assemble and use a commercially available microfluidic specimen holder. These specialized holders utilize silicon nitride microchips produced with integrated spacers to form a liquid chamber that encloses minute volumes of solution. Thin, transparent windows are etched into the microchips for imaging purposes¹². We demonstrate the proper use of a microfluidic holder to examine simian rotavirus double-layered particles (DLPs) in liquid using a TEM. To ensure that biological assemblies, such as DLPs, do not rapidly diffuse over great distances while imaging, we employ the Affinity Capture approach to tether them to the surface of the microfluidic chamber¹⁶. This molecular capture step has a major advantage over alternative techniques for imaging biological specimens in liquid because it allows for the acquisition of images that will be used for downstream processing routines. This capture step used in conjunction with microfluidic imaging is unique to our procedures¹⁷. Readers employing structural biology applications using TEM or microfluidic imaging chambers may consider the use of affinity capture techniques when dynamic observations at the molecular level are the end goal.

Protocol

1. Prepare Affinity Capture Devices¹⁶

1. Clean the silicon nitride E-chips by incubating them in 15 ml of acetone for 2 min followed by 15 ml of methanol for 2 min (Figure 1A). Allow chips to dry under laminar air-flow.
2. Incubate the dried chips on a heated stir plate (without stirring) for 1.5 hr at 150 °C, then allow them to cool to room temperature before use.
3. Use Hamilton syringes to compose lipid mixtures in small glass tubes to contain 25% chloroform, 55% DLPC (1,2-dilauroyl-phosphocholine) in chloroform (1 mg/ml) and 20% Ni-NTA lipid (1,2-dioleoyl-iminodiacetic acid-succinyl-nickel salt) in chloroform (1 mg/ml) for a total volume of 40 μl.
4. Apply a 1 μl aliquot of the mixture over each 15 μl drop of Milli-Q water on a piece of Parafilm in a humid Petri dish (Figure 1B). Incubate samples on ice for at least 60 min.

2. Capture Macromolecules¹⁷

1. Place an E-chip with a 150 nm integrated spacer on top of a monolayer sample and incubate for 1 min (Figure 1C).
2. Gently lift the chip off of the sample and add a 3 μl aliquot of His-tagged Protein A. Incubate for 1 min at room temperature (Figure 1D).
3. Blot away the excess drop using Whatman #1 filter paper and immediately add a 3 μl aliquot of antibody solution. Incubate for 1 min at room temperature.
4. Remove the excess solution using a Hamilton syringe and immediately add a 1 μl aliquot of rotavirus DLPs (0.1 mg/ml) in buffer solution containing 50 mM HEPES (pH 7.5), 150 mM NaCl, 10 mM MgCl₂ and 10 mM CaCl₂. Incubate for at least 2 min at room temperature. The preparation of DLPs has been described previously¹⁸.

3. Assemble the Microfluidic Chamber and Load the In situ Specimen Holder

1. Load the wet E-chip containing the viral sample into the tip of the microfluidic specimen holder. Glow-discharge a second flat E-chip for 1 min then loaded on top of the spacer chip (Figure 2, panels 1-5).
2. Alternatively, to increase the contrast of biological macromolecules, heavy metal stain (e.g. 0.2% uranyl formate) can be added to wet specimens prior to placing the second E-chip on the wet specimen. However, the E-chip containing the specimen will need to be washed with Milli-Q water prior to adding the contrast reagent.
3. Sandwich the entire assembly together to form a sealed enclosure, held in place mechanically within the holder by 3 brass screws (Figure 2, panels 6-8).
4. Following assembly, the tip of the holder is pumped to 10^-6 Torr using a turbo-pumped dry pumping station before placing the holder inside the TEM.

4. Imaging in Liquid Using a Transmission Electron Microscope

1. Load the in situ specimen holder into a Transmission Electron Microscope (FEI Company) equipped with a LaB₆ filament and operating at 120 kV.
2. Turn on the TEM filament and adjust the eucentric height of the microscope stage with respect to the specimen by using the wobbler function to tilt the sample from -15° to +15° back and forth in the column. This procedure adjusts the stage in the z-direction to help account for the proper thickness of the liquid chamber. This step also ensures an accurate magnification is used when recording images.
3. Record images along the edges and in the corner regions of the microfluidic chamber first. These areas typically contain the thinnest solution. Record images of specimens under low-dose conditions (1-3 electrons/Ų) using a CCD camera. Use nominal magnifications of 6,000X - 30,000X.
4. Determine the proper defocus value by focusing at the edge of fluidic chamber. Use a value of -1.5 μm to record images at 30,000X magnification. If thick solution is encountered or if contrast agent is not used in the specimen preparation, use higher defocus values in the range of -2 to -4 μm.
5. To ensure solution is contained in the microfluidic chamber throughout the experiments, focus the electron beam until the bubbles are formed in the liquid within the device.

Results

Representative images of DLPs in liquid using E-chips that were glow-discharged (Figure 3A) show fewer DLPs in a given viewing area, presumably due to diffusion, in comparison to DLPs that are enriched on Affinity Capture devices (Figure 3B). The addition of uranyl formate in the imaging chamber enhances the contrast of the specimen and hence the visibility of individual DLPs in solution (Figure 3C, top panel). Better contrast allows for downstream image p...

Discussion

In our presented work, we employed the affinity capture approach to tether rotavirus DLPs to a microfluidic platform. This allowed for in situ imaging of macromolecular complexes in a liquid microenvironment. The capture approach is significant with respect to other microfluidic imaging techniques because it localizes biological specimens to the imaging window to negate large diffusive issues that arise while recording images in liquid. However, one of the most critical steps in our protoc...

Materials

Name	Company	Catalog Number	Comments
E-chips, spacer chip	Protochips, Inc.	EPB-52TBD	400 μm x 50 μm window
E-chip, top chip	Protochips, Inc.	EPT-45W	400 μm x 50 μm window
Ni-NTA lipid	Avanti Polar Lipids	790404P	Powder form
DLPC (12:0) lipid	Avanti Polar Lipids	850335P	Powder form
Volumetric flasks	Fisher Scientific	20-812A; 20-812C	1 ml; 5 ml
Hamilton Syringes	Hamilton Co.	80300, 80400	1-10 μl; 1-25 μl
Whatman #1 filter paper	Whatman	1001 090	100 pieces, 90 mm
Glass Petri dishes	Corning	70165-101	100 mm x 15 mm
Glass Pasteur pipettes	VWR	14673-010	14673-010
Glass culture tubes	VWR	47729-566	6 mm x 50 mm
Acetone	Fisher Scientific	A11-1	1 L
Methanol	Fisher Scientific	A412-1	1 L
Chloroform	Electron Microcopy Sciences	12550	100 ml
His-tagged Protein A	Abcam, Inc.	ab52953	10 mg
Milli-Q water system	EMD Millipore Corp.	Z00QSV001	Ultrapure Water
HEPES	Fisher Scientific	BP310-500	500 g
Equipment
Poseidon In situ specimen holder	Protochips, Inc.		FEI compatible
FEI Spirit BioTwin TEM	FEI Co.		120 kV
Eagle 2k HS CCD camera	FEI Co.		10 Å/pixel sampling at 30,000X
Gatan 655 Dry pump station	Gatan, Inc.		Pump holder tip to 10-6 range
PELCO easiGlow, glow discharge unit	Ted Pella, Inc.		Negative polarity mode
Isotemp heated stir plate	Fisher Scientific		Heat to 150 ºC for 1.5 hr