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Summary

Abstract

Introduction

Protocol

Representative Results

Discussion

Acknowledgements

Materials

References

Medicine

Subclavian Vein Puncture As an Alternative Method of Blood Sample Collection in Rats

Published: November 18th, 2018

DOI:

10.3791/58499

1Department of Cardiology, The Second Xiangya Hospital, Central South University, 2Department of General Surgery, The Second Xiangya Hospital, Central South University

Here, we present a protocol to collect blood samples from the rat subclavian vein.

Blood collection with enough blood volume is essential in animal experiments. Blood collection from the tail vein of rats is popular and less stressful compared to other more aggressive methods such as retro-orbital plexus sample collection. However, this blood collection method is sometimes limited by an unsatisfactory success rate. Here, we introduce a method for blood collection through the subclavian vein puncture. The subclavian vein is located just under the clavicle and this vein is large enough to fulfill the volume requirements of blood collection. Our results show that this method is safe and applicable for blood collection sampling with the required blood volume. Blood collection through the subclavian vein puncture could serve as an alternative blood collection method in case of failed tail vein blood sampling in rats.

Blood collection is essential in animal research. The target veins for blood collection include the retro-orbital plexus, the jugular vein, the saphenous vein, tail blood vessels, and the carotid artery1,2,3,4. Sometimes, blood could be obtained from the abdominal aorta, vena cava or even the heart5,6,7. On such occasions, the animals must be sacrificed and cannot be used for subsequent observation; thus, these methods are used less in daily experim....

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This study was approved by the Central South University Ethics Committee for Animal Research from the Second Xiangya Hospital (Changsha, China). The manuscript was prepared according to ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines9.

1. Material and Animal Preparation

  1. Prepare required materials: adhesive tape, epilating agent, 75% ethanol, blood collection tube, 2.5 mL syringe connected with needle (24 G, 0.6 mm x 25 mm), hair shaver,.......

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A total of 20 SD rats (male n=10, female n=10) were used here. Blood collectionwas performed by skilled operators, who have performed more than 20 blood samplings from the subclavian vein in rats, and by the beginner operators, who have no subclavian vein puncture experience in rats or other animals. Before operations, beginners watched at least 3 procedures done by skilled operators.

Blood sampling succeeded in 17 rats thr.......

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Here, we introduce an alternative method for blood extraction from the subclavian vein and we show that this method is feasible, safe, and applicable for blood sampling in rats. This method is derived from the subclavian vein puncture in pacemaker lead implantation for patients11. The subclavian vein is located just posterior and caudal to the clavicle and is a continuation of the axillary vein. It runs from the outer border of the first rib to the medial border of anterior scalene muscle

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None declared.

....

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Name Company Catalog Number Comments
rats Hunan SJA Laboratory Animal Co., Ltd (Changsha, Hunan Province, China) 
stastical software SPSS Statistics 17
epilating agent France Yi Sha Cosmetics Co.,Ltd(Guangzhou, Guangdong Province ,China)
2.5 mL syringe Shandong Weigao Group Medical Polymer Co.,Ltd(Weihai,Shandong Province ,China)
hair shaver Shanghai FLYCO Electric Co., Ltd(Shanghai,China)
adhesive tape 3M Deutschland GmbH(EdisonstraBe 6,59157 Kamen, Germany)
Pentobarbital sodium Merck
75% ethanol Department of Pharmacy,The Second Xiangya Hospital of Central South Univesity
blood collection tube Hubei Jinxing Technology&Development Co.,Ltd (Wuhan Hubei Province,China) (2ml)
electronic scale Dongguan Shengheng Electronics Co.,Ltd (Dongguan,Guangdng Province,China)
canvas gloves for anethesia
hepain Nanjing Xinbai Pharmaceutical  Co.,Limited (Nanjing, Jiangsu Province, China) (2mL, 12500 IU)
physical saline Hunan Kelun Pharmaceutical Co., Ltd(Yueyang ,Hunan Province,China) (100ml)

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