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Abstract

Medicine

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

Published: November 2nd, 2019

DOI:

10.3791/59983

1Department of General, Visceral, and Transplant Surgery, Ludwig Maximilian University of Munich, 2Walter-Brendel-Centre of Experimental Medicine, Ludwig Maximilian University of Munich, 3Department of General, Visceral and Cancer Surgery, University Hospital of Cologne, University of Cologne

Abstract

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. However, only minor improvement in the long-term results of transplanted solid organs could be observed over the past decades. In this context, chronic allograft vasculopathy (CAV) still represents the leading cause of late organ failure in cardiac, renal and pulmonary transplantation.

Thus far, the underlying pathogenesis of CAV development remains unclear, explaining why effective treatment strategies are presently missing and emphasizing a need for relevant experimental models in order to study the underlying pathophysiology leading to CAV formation. The following protocol describes a murine heterotopic cervical aortic transplantation model using a modified non-suture cuff technique. In this technique, a segment of the thoracic aorta is interpositioned in the right common carotid artery. With the use of the non-suture cuff technique, an easy to learn and reproducible model can be established, minimizing the possible heterogeneity of sutured vascular micro anastomoses.

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Keywords Murine

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