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Abstract

Biology

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published: December 22nd, 2020

DOI:

10.3791/62008

1The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York, 2The City College of New York, CUNY, 3The Bronx High School of Science, 4The Graduate Center - Advanced Science Research Center, Structural Biology Initiative, The City University of New York, 5The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York

* These authors contributed equally

Abstract

Metabolomics, the study to identify and quantify small molecules and metabolites present in an experimental sample, has emerged as an important tool to investigate the biological activities during development and diseases. Metabolomics approachesĀ are widely employed in the study of cancer, nutrition/diet, diabetes, and other physiological and pathological conditions involving metabolic processes. An advantageous tool that aids in metabolomic profiling advocated in this paper is matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Its ability to detect metabolites in situ without labeling, structural modifications, or other specialized reagents, such as those used in immunostaining, makes MALDI MSI a unique tool in advancing methodologies relevant in the field of metabolomics. An appropriate sample preparation process is critical to yield optimal results and will be the focus of this paper.

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