This work was supported by the Deutsche Forschungsgemeinschaft (DU 1190/3-1).

Tüm hayvan deneyleri Alman hayvan koruma kanunu (TierSchG) uygun olarak yapılmıştır. FELASA (www.felasa.eu/guidelines.php) ve ulusal hayvan refahı vücut GV-SOLAS (www.gv-solas.de/index.html) tarafından tanımlanan fareler ev sahipliği ve iyi hayvan uygulamalarına uygun olarak ele alındı. Freiburg Üniversitesi hayvan refahı komitesi yanı sıra yerel yönetimler (Regierungspräsidium Freiburg) tüm hayvan deneyleri onayladı. 1. Hayvan Taşıma, hazırlanması ve Enflamasyon indüksiyonu <ol><li> 20 g - 16 ağırlık aralığı ile 6 haftalık erkek fareler - 4 kullanın. Not: fareler eski veya daha ağır olmaları durumunda, mikroskobik gözlem sınırlayan, damar etrafındaki aşırı yağ sunuyoruz. </li><li> Ameliyat öncesinde tüm araçları ve mikroskop tablosunu sterilize edin. </li><li> Enjekte 20 mg / kg LPS (lipopolisakarit) intraperitonal olarak 4 bakteriyel Inflamm uyarılması için bir oturma fare içine mikroskop önce saattirme. </li><li> Plastik odası ve mezenterik doku nemlendirmek için 37 ° C sıcaklıkta bir su banyosu içinde bir% 0.9 tuzlu su çözeltisi Prewarm. </li><li> Sağ mikroskopi işlem öncesi intraperitonal ketamin enjekte (100 mg / kg) ve ksilazin (5 mg / kg) ile fare anestezisi. Alternatif anestezi, örneğin,% 2 izofluran teneffüs kullanılabilir. Refleks uyarım (firma basınç ayak veya kuyruk tutam) yanıt kaybı ile doğru anesthetization onaylayın. </li><li> Bir tıraş makinesi kullanarak karın atmaktadır ve etanol% 70 doymuş gazlı bez ile gevşek saç çıkarın. </li><li> Anestezi altında iken, kuruluk önlemek için fare gözleri veteriner merhem sürün. </li></ol> 2. Cerrahi <ol><li> % 70 etanol kullanılarak karın sterilize edin. Bu yöntem, steril bir yöntem değildir, deney sonunda öldürücü olabilir. </li><li> Median laparotomi gerçekleştirin: küçük, kavisli bir forseps ve küçük bir makas kullanarak karın cildi açın. Epi tanımlayıngastrical gemiler damarları korumak için, linea alba bölgesinde periton açın ve. </li><li> Nemli doku tutmak için karın boşluğuna önceden ısıtılmış tuzlu su bir kaç damla uygulanır. </li><li> Floresan 50 ul rodamin 6G 9,10 daha önce tanımlandığı gibi, retro-orbital (1 mg / mi) enjekte edilmesiyle Etiket lökositlerin ve trombositlerin. </li><li> 10 cm çapında bir petri içinde ileumand yerinde bunun bir döngü exteriorize ve 37 ° C uygulayarak nemli doku tutmak için emin olun tuzlu su çözeltisi (% 0.9) her dakika önceden ısıtılmış. </li></ol> 3. Intravital Mikroskopi <ol><li> Mikroskop altına Fare yerleştirin ve 200 çapında mezenter ven getirmek - görünümünde merkezinde 300 um. Hiçbir görünür yağ çevresi ile bir gemi seçin. </li><li> Böylece endotel uyarılmasını önlemek mezenter damarlarını dokunmayın. Dikkatli ileum döngü Kolu. </li><li> Kan hücresi endotel etkileşimlerini görselleştirinBir ters çevrilmiş veya dik bir mikroskop ve mikroskop yazılımı kullanarak bir kamera ile. Tutanak kan fare başına 4 farklı damarlarda 1 dakika için hücre-endotelyal etkileşimleri. </li><li> Görüntüleme Deneylerin tamamlanmasından sonra, servikal dislokasyon ile fare öldürülür. </li></ol> 4. Analiz <ol><li> Analiz çevrimdışı yürütmek ve tüm parametreler için kör. Herhangi bir uygun yazılım programı kullanarak analiz gerçekleştirin. </li><li> Intraluminal kan hücresi akımı odaklanmış yüksek zaman çözünürlüklü sine-klipleri istikrarlı ve interindividually karşılaştırılabilir kan akımı koşulları (maksimal kare hızı) onaylayın. </li><li> Haddeleme lökosit sayısını ölçmek. Bunun damar yoluyla dikey bir çizgi çizin ve 1 dakika içinde bu çizgiyi tüm lökositlerin sayısı. </li><li> Tek lökosit stabil endotel üzerinde yuvarlanma sırasında 50 mikron mesafe geçmek gerekiyor zamanı ölçerek haddeleme hızı belirleyin. Bunu yapmak için, 50 um t mesafede iki dikey çizgi çizinven hrough. <ol><li> Temsili bir lökosit gerekli zaman (mm / s) üzerinden 50 mikron bölerek other.Calculate bir hattan lökositlerin hız almak için gereken süreyi ölçün. </li></ol></li><li> 0.04 mm&#39;lik bir 2 alanda lökosit adezyonu ölçün. Bunu yapmak için, damara 200 mikron yan uzunluğunda bir kare çizin. <ol><li> Bu kare içinde 30 saniye boyunca hiçbir görünür hareket olarak tanımlanan firma yapışık lökositler, Kont. </li></ol></li><li> Platelet lökosit etkileşimlerini ölçmek için tek lökosit bağlı trombositlerin sayısını. </li></ol>

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	<li>Lehr, H. A., Vollmar, B., Vajkoczy, P., Menger, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravital+fluorescence+microscopy+for+the+study+of+leukocyte+interaction+with+platelets+and+endothelial+cells.">Intravital fluorescence microscopy for the study of leukocyte interaction with platelets and endothelial cells.</a> Methods in enzymology. 300, 462-481 (1999).</li><li>Atherton, A., Born, G. 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K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ADAMTS13:+a+new+link+between+thrombosis+and+inflammation.">ADAMTS13: a new link between thrombosis and inflammation.</a> The Journal of experimental medicine. 205, 2065-2074 (1084).</li><li>Baez, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+open+cremaster+muscle+preparation+for+the+study+of+blood+vessels+by+in+vivo+microscopy.">An open cremaster muscle preparation for the study of blood vessels by in vivo microscopy.</a> Microvascular research. 5, 384-394 (1973).</li><li>Leister, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+peritoneal+cavity+chamber+for+intravital+microscopy+of+the+liver+under+conditions+of+pneumoperitoneum.">A peritoneal cavity chamber for intravital microscopy of the liver under conditions of pneumoperitoneum.</a> Surgical endoscopy. 17, 939-942 (2003).</li><li>Duerschmied, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Platelet+serotonin+promotes+the+recruitment+of+neutrophils+to+sites+of+acute+inflammation+in+mice.">Platelet serotonin promotes the recruitment of neutrophils to sites of acute inflammation in mice.</a> Blood. 121, 1008-1015 (2013).</li><li>Herr, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+fluoxetine+treatment+induces+slow+rolling+of+leukocytes+on+endothelium+in+mice.">Acute fluoxetine treatment induces slow rolling of leukocytes on endothelium in mice.</a> PloS one. 9, e88316 (2014).</li><li>Yardeni, T., Eckhaus, M., Morris, H. 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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+vessel+wall+and+its+interactions.">The vessel wall and its interactions.</a> Blood. 111, 5271-5281 (2008).</li><li>Gawaz, M., Fateh-Moghadam, S., Pilz, G., Gurland, H. 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The authors have nothing to disclose.

Bu yazıda hazırlık ve in vivo farelerin mezenter damarlarının intravital mikroskopi performansını açıklamak. Bu yöntem bize canlı organizmada doğrudan lökosit-endotel ve trombosit endotel etkileşimleri 11 gözlemleme fırsatı veriyor. Endotel inflamasyon için erken yanıt olarak aktive olur ve haddeleme, yapışma ve göçüne 12 sonuçlanan lökositler ve trombositler ile etkileşime girer. Ancak lökositler de trombosit sözde şekillendirme i...

Bu protokolün amacı, inflamatuvar koşullarda lökosit-endotel ve lökosit-trombosit etkileşimleri doğrudan gözlem için fareler yaşayan mezenter damarların intravital mikroskopi basitleştirilmiş tekniği tanımlamaktır. Intravital mikroskopi inflamatuar lökosit işe alma ve işlevini anlamak için önemsiz ama önemli değil enflamatuvar durumlarda 1,2 altında in vivo lökosit-endotel etkileşimleri incelemek için geliştirilmiştir. Bu protokolde biz tarif yöntemi daha önce yayınlanan yayınlara göre geliştirilmiştir. Yayımlanmıştır ne büyüyen trombüs 3 ve paralel olarak trombosit birleşmesini görselleştirmek benzer daha erken 4, bir batın mezenterik ven iletilen ışık mikroskobu ile incelenir. Bu tür sıçanlarda 5 veya fare karaciğer ve sıçanların 6 cremaster kas gibi içi hayati mikroskobu çeşitli modelleri vardır. Mezenter ven intravital mikroskopi geliştirilmiş ve birçok grup ile daha önceki çalışmalarda uygulanmıştır. / - - Kimin trombosit serotonin farmakolojik uzun vadeli tarafından tükenmiş farelere bulgular nöronal olmayan serotonin eksikliği ve karşılaştırılmıştır olan fareler, vahşi tip fareler ve triptofan hydroxylase1 (TPH1) arasında lökosit işe farklılıkları gözlemlemek için bu tekniği kullandık Uygulama, bir seçici serotonin tekrar-yükselme inhibitörü, fluoksetin 7. Biz de akut fluoksetin tedavisinin 8. sonrası lökosit-endotel etkileşimleri incelenmiştir. Bu model, hızla gerçekleştirilen ve lökosit-endotel ve trombosit lökosit etkileşimleri geçerli ölçümleri sağlar, çünkü bu protokolde, mezenter damarların intravital mikroskopi odaklanmak. Bu çok daha zor ve zaman alıcı kemik, karaciğer, deri, ya da cremaster kas gibi diğer organlara, intravital mikroskobiyle bulunmaktadır. kipBurada tarif edilen L, lipopolisakarit intraperitoneal enjeksiyonu gibi bir enflamatuar uyarıcı, ile challengeing sonrası inflamatuar hücre-hücre etkileşim tekrarlanabilir değerlendirilmesi için idealdir.

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	<tbody>
		<tr>
			<td>Rhodamine 6G</td>
			<td>Sigma-Aldrich</td>
			<td>&#160;989-38-8</td>
			<td></td>
		</tr>
		<tr>
			<td>Lipopolysaccharides from Escherichia coli 055:B5</td>
			<td>Sigma-Aldrich</td>
			<td>L2637&#160;</td>
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intravital microscopy of leukocyte-endothelial and platelet-leukocyte interactions in mesenterial veins in mice

Intravital mikroskopi yaşayan hayvanlarda farklı farklı bölgelerde süreçleri ve damarları araştırmak için kullanılabilecek bir yöntemdir. Bu protokol, biz mezenter damarların intravital mikroskobu açıklar. Bu, in vivo olarak, lökosit-endoteliyal etkileşimleri gösteren tekrarlanabilir sonuçlar ile kısa bir süre içinde gerçekleştirilebilir. Biz endotel LPS mücadeleden sonra inflamatuar bir ayarı açıklamak. Ancak bu modelde biri bakteriyel kimyasal veya biyolojik gibi iltihaplı durumların birçok farklı türde uygulamak ve ilaçların yönetimini ve yaşam hayvan üzerindeki doğrudan etkileri ve lökosit işe üzerindeki etkisini araştırmak yapabilirsiniz. Bu protokol, farelerde ve damarlarda inflamatuar yanıt üzerindeki etkileri farklı tedaviler için başarılı bir şekilde uygulanmıştır. Burada, floresan rodamin 6G bu etiketleme yoluyla lökosit ve trombosit görselleştirme açıklar. Buna ek olarak, herhangi bir görüntüleme pe olabilirhedeflenen floresan etiketli moleküller kullanılarak rformed.

Bu protokolde tarif edilen deneysel ortamda bir genel görünüşü Şekil 1&#39;de gösterilmiştir. Bu intravital mikroskobiyle gözlenebilir bir batın ileum-döngü ve kaplar, bir fare göstermektedir. Şekil 2 nedeniyle işlem görmemiş hayvanlarda aktivasyon belli ölçüde göstermektedir prosedür kendisi. Fakat hemen hemen hiç yavaş haddeleme ya LPS ile tedavi edilen farelere kıyasla, lökositlerin Sıkı yapışma vardır. Ayrıca, vahşi tipli fare ya içme suyu ya da ted...

Watch this Scientific Journal Video about Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice at JoVE.com

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.

Farelerde mesenteriumda Damarlardaki intravital Mikroskopi Lökosit-endotelyal ve Trombosit-lökosit Etkileşimleri

Intravital microscopy is a method that can be used to investigate different processes in different regions and vessels in living animals. In this ...

intravital-microscopy-leukocyte-endothelial-platelet-leukocyte

Research

JoVE Journal

Immunology and Infection

12.0K Görüntüleme.  Heart Center, University of Freiburg. This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.

Video: Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Intravital Microscopy of the Inguinal Lymph Node

Lymph nodes (LN's), located throughout the body, are an integral component of the immune system. They serve as a site for induction of adaptive immune response and therefore, the development of effector cells. As such, LNs are key to fighting invading pathogens and maintaining health. The choice of LN to study is dictated by accessibility and the desired model; the inguinal lymph node is well situated and easily supports studies of biologically relevant models of skin and genital mucosal infection.
The inguinal LN, like all LNs, has an extensive microvascular network supplying it with blood. In general, this microvascular network includes the main feed arteriole of the LN that subsequently branches and feeds high endothelial venules (HEVs). HEVs are specialized for facilitating the trafficking of immune cells into the LN during both homeostasis and infection. How HEVs regulate trafficking into the LN under both of these circumstances is an area of intense exploration. The LN feed arteriole, has direct upstream influence on the HEVs and is the main supply of nutrients and cell rich blood into the LN. Furthermore, changes in the feed arteriole are implicated in facilitating induction of adaptive immune response. The LN microvasculature has obvious importance in maintaining an optimal blood supply to the LN and regulating immune cell influx into the LN, which are crucial elements in proper LN function and subsequently immune response. 
The ability to study the LN microvasculature in vivo is key to elucidating how the immune system and the microvasculature interact and influence one another within the LN. Here, we present a method for in vivo imaging of the inguinal lymph node. We focus on imaging of the microvasculature of the LN, paying particular attention to methods that ensure the study of healthy vessels, the ability to maintain imaging of viable vessels over a number of hours, and quantification of vessel magnitude. Methods for perfusion of the microvasculature with vasoactive drugs as well as the potential to trace and quantify cellular traffic are also presented. 
Intravital microscopy of the inguinal LN allows direct evaluation of microvascular functionality and real-time interface of the direct interface between immune cells, the LN, and the microcirculation. This technique potential to be combined with many immunological techniques and fluorescent cell labelling as well as manipulated to study vasculature of other LNs.

A technique for performing intravital microscopy of the inguinal lymph node (LN) is outlined. Such technique allows for real-time, in vivo study of the lymph node microvasculature and structure both during homeostasis and infection. This technique can be adapted to cell trafficking studies and to other lymph node sites.

Kasık lenf düğüm intravital Mikroskopi

Lymph nodes (LN's), located throughout the body, are an integral component of the immune system. They serve as a site for induction of adaptive immune ...

İmmünoloji ve Enfeksiyon

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

The recruitment of circulating leukocytes from blood stream to the inflamed tissue is a crucial and complex process of inflammation1,2. In the postcapillary venules of inflamed tissue, leukocytes initially tether and roll on the luminal surface of venular wall. Rolling leukocytes arrest on endothelium and undergo firm adhesion in response to chemokine or other chemoattractants on the venular surface. Many adherent leukocytes relocate from the initial site of adhesion to the junctional extravasation site in endothelium, a process termed intraluminal crawling3. Following crawling, leukocytes move across endothelium (transmigration) and migrate in extravascular tissue toward the source of chemoattractant (chemotaxis)4. Intravital microscopy is a powerful tool for visualizing leukocyte-endothelial cell interactions in vivo and revealing cellular and molecular mechanisms of leukocyte recruitment2,5. In this report, we provide a comprehensive description of using brightfield intravital microscopy to visualize and determine the detailed processes of neutrophil recruitment in mouse cremaster muscle in response to the gradient of a neutrophil chemoattractant. To induce neutrophil recruitment, a small piece of agarose gel (~1-mm3 size) containing neutrophil chemoattractant MIP-2 (CXCL2, a CXC chemokine) or WKYMVm (Trp-Lys-Tyr-Val-D-Met, a synthetic analog of bacterial peptide) is placed on the muscle tissue adjacent to the observed postcapillary venule. With time-lapsed video photography and computer software ImageJ, neutrophil intraluminal crawling on endothelium, neutrophil transendothelial migration and the migration and chemotaxis in tissue are visualized and tracked. This protocol allows reliable and quantitative analysis of many neutrophil recruitment parameters such as intraluminal crawling velocity, transmigration time, detachment time, migration velocity, chemotaxis velocity and chemotaxis index in tissue. We demonstrate that using this protocol, these neutrophil recruitment parameters can be stably determined and the single cell locomotion conveniently tracked in vivo.

We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.

Takip Nötrofil Lumen Crawling, intravital video Mikroskopi Doku transendoteliyal Göç ve Kemotaksis

The recruitment of circulating leukocytes from blood stream to the inflamed tissue is a crucial and complex process of inflammation1,2. In the ...

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Two-photon Microscopy (TPM) provides image acquisition in deep areas inside tissues and organs. In combination with the development of new stereotactic tools and surgical procedures, TPM becomes a powerful technique to identify "niches" inside organs and to document cellular "behaviors" in live animals. While intravital imaging provides information that best resembles the real cellular behavior inside the organ, it is both more laborious and technically demanding in terms of required equipment/procedures than alternative ex vivo imaging acquisition. Thus, we describe a surgical procedure and novel "stereotactic" organ holder that allows us to follow the movements of Foxp3+ cells within the thymus.
Foxp3 is the master regulator for the generation of regulatory T cells (Tregs). Moreover, these cells can be classified according to their origin: ie. thymus-differentiated Tregs are called "naturally-occurring Tregs" (nTregs), as opposed to peripherally-converted Tregs (pTregs). Although significant amount of research has been reported in the literature concerning the phenotype and physiology of these T cells, very little is known about their in vivo interactions with other cells. This deficiency may be due to the absence of techniques that would permit such observations. The protocol described in this paper provides a remedy for this situation.
Our protocol consists of using nude mice that lack an endogenous thymus since they have a punctual mutation in the DNA sequence that compromises the differentiation of some epithelial cells, including thymic epithelial cells. Nude mice were gamma-irradiated and reconstituted with bone marrows (BM) from Foxp3-KIgfp/gfp mice. After BM recovery (6 weeks), each animal received embryonic thymus transplantation inside the kidney capsule. After thymus acceptance (6 weeks), the animals were anesthetized; the kidney containing the transplanted thymus was exposed, fixed in our organ holder, and kept under physiological conditions for in vivo imaging by TPM. We have been using this approach to study the influence of drugs in the generation of regulatory T cells.

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of &#x201C;niches&#x201D; within the thymus where T cell development occurs.

2-Foton Mikroskopi kullanarak Fare Thymus intravital Görüntüleme

Two-photon Microscopy (TPM) provides image acquisition in deep areas inside tissues and organs. In combination with the development of new stereotactic ...

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

The advent of intravital microscopy in experimental rodent malaria models has allowed major advances to the knowledge of parasite-host interactions 1,2. Thus, in vivo imaging of malaria parasites during pre-erythrocytic stages have revealed the active entrance of parasites into skin lymph nodes 3, the complete development of the parasite in the skin 4, and the formation of a hepatocyte-derived merosome to assure migration and release of merozoites into the blood stream 5. Moreover, the development of individual parasites in erythrocytes has been recently documented using 4D imaging and challenged our current view on protein export in malaria 6. Thus, intravital imaging has radically changed our view on key events in Plasmodium development. Unfortunately, studies of the dynamic passage of malaria parasites through the spleen, a major lymphoid organ exquisitely adapted to clear infected red blood cells are lacking due to technical constraints.
Using the murine model of malaria Plasmodium yoelii in Balb/c mice, we have implemented intravital imaging of the spleen and reported a differential remodeling of it and adherence of parasitized red blood cells (pRBCs) to barrier cells of fibroblastic origin in the red pulp during infection with the non-lethal parasite line P.yoelii 17X as opposed to infections with the P.yoelii 17XL lethal parasite line 7. To reach these conclusions, a specific methodology using ImageJ free software was developed to enable characterization of the fast three-dimensional movement of single-pRBCs. Results obtained with this protocol allow determining velocity, directionality and residence time of parasites in the spleen, all parameters addressing adherence in vivo. In addition, we report the methodology for blood flow quantification using intravital microscopy and the use of different colouring agents to gain insight into the complex microcirculatory structure of the spleen. 
Ethics statement
All the animal studies were performed at the animal facilities of University of Barcelona in accordance with guidelines and protocols approved by the Ethics Committee for Animal Experimentation of the University of Barcelona CEEA-UB (Protocol No DMAH: 5429). Female Balb/c mice of 6-8 weeks of age were obtained from Charles River Laboratories.

We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

Dalak intravital Mikroskopi: Parazit Mobilite ve Kan Akımının Kantitatif Analiz

The advent of intravital microscopy in experimental rodent malaria models has allowed major advances to the knowledge of parasite-host interactions 1,2. ...

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Monitoring cellular communication by intravital deep-tissue multi-photon microscopy is the key for understanding the fate of immune cells within thick tissue samples and organs in health and disease. By controlling the scanning pattern in multi-photon microscopy and applying appropriate numerical algorithms, we developed a striped-illumination approach, which enabled us to achieve 3-fold better axial resolution and improved signal-to-noise ratio, i.e. contrast, in more than 100 &#181;m tissue depth within highly scattering tissue of lymphoid organs as compared to standard multi-photon microscopy. The acquisition speed as well as photobleaching and photodamage effects were similar to standard photo-multiplier-based technique, whereas the imaging depth was slightly lower due to the use of field detectors. By using the striped-illumination approach, we are able to observe the dynamics of immune complex deposits on secondary follicular dendritic cells &#8211; on the level of a few protein molecules in germinal centers.

High-resolution intravital imaging with enhanced contrast up to 120 &#181;m depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 &#181;m depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.

Germinal Merkezleri derece Çözülmüş Intravital Çizgili-aydınlatma Mikroskopi

Monitoring cellular communication by intravital deep-tissue multi-photon microscopy is the key for understanding the fate of immune cells within thick ...

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Liver inflammation as a response to injury is a highly dynamic process involving the infiltration of distinct subtypes of leukocytes including monocytes, neutrophils, T cell subsets, B cells, natural killer (NK) and NKT cells. Intravital microscopy of the liver for monitoring immune cell migration is particularly challenging due to the high requirements regarding sample preparation and fixation, optical resolution and long-term animal survival. Yet, the dynamics of inflammatory processes as well as cellular interaction studies could provide critical information to better understand the initiation, progression and regression of inflammatory liver disease. Therefore, a highly sensitive and reliable method was established to study migration and cell-cell-interactions of different immune cells in mouse liver over long periods (about 6 hr) by intravital two-photon laser scanning microscopy (TPLSM) in combination with intensive care monitoring.
The method provided includes a gentle preparation and stable fixation of the liver with minimal perturbation of the organ; long term intravital imaging using multicolor multiphoton microscopy with virtually no photobleaching or phototoxic effects over a time period of up to 6 hr, allowing tracking of specific leukocyte subsets; and stable imaging conditions due to extensive monitoring of mouse vital parameters and stabilization of circulation, temperature and gas exchange.
To investigate lymphocyte migration upon liver inflammation CXCR6.gfp knock-in mice were subjected to intravital liver imaging under baseline conditions and after acute and chronic liver damage induced by intraperitoneal injection(s) of carbon tetrachloride (CCl4).
CXCR6 is a chemokine receptor expressed on lymphocytes, mainly on Natural Killer T (NKT)-, Natural Killer (NK)- and subsets of T lymphocytes such as CD4 T cells but also mucosal associated invariant (MAIT) T cells1. Following the migratory pattern and positioning of CXCR6.gfp+ immune cells allowed a detailed insight into their altered behavior upon liver injury and therefore their potential involvement in disease progression.

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

CXCR6.Gfp Muhabir Fare kullanma Sağlıklı ve hastalıklı Karaciğer Bağışıklık Hücreleri Uzun Vadeli intravital multiphoton Mikroskopi Görüntüleme

Liver inflammation as a response to injury is a highly dynamic process involving the infiltration of distinct subtypes of leukocytes including monocytes, ...

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site of infection or injury. Investigating leukocyte migration in vivo is crucial for understanding the molecular basis of leukocyte transendothelial migration and interaction with vascular endothelium. One powerful approach involves intravital microscopy on transgenic mice expressing fluorescent proteins in cells of interest.
Here we present a protocol for imaging monocytes and neutrophils in the CX3CR1gfp/wt mouse i.v. injected with orange dye-labeled neutrophils with an inverted confocal microscope. Time-lapse movies gathered from 30 min&#160;to several hours of imaging allow the analysis of leukocyte behavior in mesenteric veins under both steady state and inflammatory conditions. We also describe the steps to locally induce blood vessel inflammation with TLR2/TLR1 agonist Pam3SK4 and monitor the subsequent recruitment of neutrophils and monocytes.
The presented technique can also be used to monitor other populations of leukocytes and investigate molecules implicated in leukocyte recruitment or trafficking using other stimuli or transgenic mice.

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

Real Time Anestetize Farelerde Intravital Mikroskopi Mezenterik Damarlardaki nötrofiller ve monositler Görüntüleme

Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site of infection or injury. Investigating leukocyte migration in ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, including cell biology, oncology, dentistry, and immunology. The SMG is an exocrine gland comprised of secretory epithelial cells, myofibroblasts, endothelial cells, nerves, and extracellular matrix. Dynamic cellular processes in the rat and mouse SMG have previously been imaged, mostly using inverted multi-photon microscope systems. Here, we describe a straightforward protocol for the surgical preparation and stabilization of the murine SMG in anesthetized mice for in vivo imaging with upright multi-photon microscope systems. We present representative intravital image sets of endogenous and adoptively transferred fluorescent cells, including the labeling of blood vessels or salivary ducts and second harmonic generation to visualize fibrillar collagen. In sum, our protocol allows for surgical preparation of mouse salivary glands in upright microscopy systems, which are commonly used for intravital imaging in the field of immunology.

We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.

Dik Intravital mikroskopi için fare Submandibular Tükürük bezi hazırlanması

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, ...

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics of the immune response to infection. Two-photon intravital microscopy (2P-IVM) allows the observation of cell interactions in deep tissue in living animals, while minimizing the photobleaching generated during image acquisition. To date, different models for 2P-IVM of lymphoid and non-lymphoid organs have been described. However, imaging of respiratory organs remains a challenge due to the movement associated with the breathing cycle of the animal.
Here, we describe a protocol to visualize in vivo immune cell interactions in the trachea of mice infected with influenza virus using 2P-IVM. To this purpose, we developed a custom imaging platform, which included the surgical exposure and intubation of the trachea, followed by the acquisition of dynamic images of neutrophils and dendritic cells (DC) in the mucosal epithelium. Additionally, we detailed the steps needed to perform influenza intranasal infection and flow cytometric analysis of immune cells in the trachea. Finally, we analyzed neutrophil and DC motility as well as their interactions during the course of a movie. This protocol allows for the generation of stable and bright 4D images necessary for the assessment of cell-cell interactions in the trachea.

In this study, we present a protocol to perform two-photon intravital imaging and cell interaction analysis in the murine tracheal mucosa after infection with influenza virus. This protocol will be relevant for researchers studying immune cell dynamics during respiratory infections.

İki fotonlu Intravital mikroskobu kullanılarak grip virüsü enfeksiyonu sırasında trakeal mukozasında görüntüleme hücre etkileşimi

The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics of the immune response to infection. ...

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal epithelium. Due to their closed structures and significant supporting extracellular matrix, 3D cultures are not ideal for host-pathogen studies. Enteroids or colonoids can be grown as epithelial monolayers on permeable tissue culture membranes to allow manipulation of both luminal and basolateral cell surfaces and accompanying fluids. This enhanced luminal surface accessibility facilitates modeling bacterial-host epithelial interactions such as the mucus-degrading ability of enterohemorrhagic E. coli (EHEC) on colonic epithelium. A method for 3D culture fragmentation, monolayer seeding, and transepithelial electrical resistance (TER) measurements to monitor the progress towards confluency and differentiation are described. Colonoid monolayer differentiation yields secreted mucus that can be studied by the immunofluorescence or immunoblotting techniques. More generally, enteroid or colonoid monolayers enable a physiologically-relevant platform to evaluate specific cell populations that may be targeted by pathogenic or commensal microbiota.

Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

Patojenler, Commensals ve ana bilgisayar bağırsak epitel arasındaki etkileşimler çalışmaya insan Colonoid Monolayers

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal ...