The general goal of this video is to introduce a new simple method to isolate and culture primary human epidermal cells from an adult skin tissue. This advanced method is more suitable for producing a large number of high-potential epidermal cells
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Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.