Cell Preparation for Live Signaling Imaging In Vivo
6:49
Orthotopic Implantation
8:28
Results: Live Single Cell Imaging In Vitro and Live TGF-β Signaling Imaging In Vivo
10:15
Conclusion
Transkript
This method can hep answer key questions in the cell signaling field by noninvasively tracking cell signaling in real time. The main advantage of this technique is that adenovirus systems provide a quick and easy method for cell signaling detectio
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Here, we present a protocol for live cell imaging of TGF-β/Smad3 signaling activity using an adenovirus reporter system. This system tracks transcriptional activity in real-time and can be applied to both single cells in vitro and in live animalmodels.