Measurement of Fluorescence and Limits of Detection
2:46
Detection and Quantification of 2 -FL in a Producer Strain
4:58
Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase
6:04
Selective Removal of Lactose: Pre-treatment with LacZ+ Strain
6:48
Selective Removal of Lactose: Lactose Precipitation with Ethanol
7:41
Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors
9:37
Conclusion
Transkript
This method can help answer several key questions involved in the production of human milk oligosaccharides. First, how much of the product do I have? And second, what is the specific carbohydrate linkage?
The goal here is to simplify and sp
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
We describe here the high-throughput detection and quantification of fucosylated human milk oligosaccharides (HMOs) using a whole-cell biosensor. We also demonstrate here, the adaptation of this platform towards analysis of HMO production strains, focusing on improving the signal to noise ratio.