Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

6.1K Views

10:17 min

April 13th, 2019

DOI :

10.3791/59253-v

April 13th, 2019


Transcript

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Human Milk Oligosaccharides

Chapters in this video

0:04

Title

1:12

Cell Culture

1:53

Measurement of Fluorescence and Limits of Detection

2:46

Detection and Quantification of 2 -FL in a Producer Strain

4:58

Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase

6:04

Selective Removal of Lactose: Pre-treatment with LacZ+ Strain

6:48

Selective Removal of Lactose: Lactose Precipitation with Ethanol

7:41

Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors

9:37

Conclusion

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