Measurement of Fluorescence and Limits of Detection
2:46
Detection and Quantification of 2 -FL in a Producer Strain
4:58
Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase
6:04
Selective Removal of Lactose: Pre-treatment with LacZ+ Strain
6:48
Selective Removal of Lactose: Lactose Precipitation with Ethanol
7:41
Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors
9:37
Conclusion
필기록
This method can help answer several key questions involved in the production of human milk oligosaccharides. First, how much of the product do I have? And second, what is the specific carbohydrate linkage?
The goal here is to simplify and sp
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We describe here the high-throughput detection and quantification of fucosylated human milk oligosaccharides (HMOs) using a whole-cell biosensor. We also demonstrate here, the adaptation of this platform towards analysis of HMO production strains, focusing on improving the signal to noise ratio.