Results: Cutting Efficiency, and Generation and Screening of Mutations
8:10
Conclusion
Transkript
The study of gene function and disease are hampered by the outbred nature of the human population. The different genetic backgrounds of patient-specific and control iPS cell lines can affect differentiation efficiency and phenotypes found in a giv
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This protocol provides a method to facilitate the generation of defined heterozygous or homozygous nucleotide changes using CRISPR-CAS9 in human pluripotent stem cells.