Results: Histone Dimers, Tetramers, Octamers, and Assembled his Tagged and TEV Digested Nucleosomes
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Conclusion
Transkript
Obtaining modified mononucleosome is crucial for designing epigenetic experiments. Our protocol provides acetylated reconstituted mononucleosomes that can be used in biochemical and binding assays, structure determination, and more. The primary ad
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Here we present a method to express acetylated histone proteins using genetic code expansion and assemble reconstituted nucleosomes in vitro.