Calibration of Polarographic Oxygen Sensors for High-Resolution Respirometry (HRR)
1:56
Dissection and Permeabilization of the Sciatic Nerve
2:58
Oxygen Consumption and Reactive Oxygen Species (ROS) Production Determination
5:51
Results: Assessing the Mitochondrial Oxygen Consumption by the Permeabilized Sciatic Nerve
7:41
Conclusion
Transkript
This protocol allows the evaluation of mitochondrial function inside of nerve by parcellation of the isolated mitochondria, a challenging procedure in a small tissue that contains a limited number of mitochondria. The technique provides a possibil
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High-resolution respirometry coupled to fluorescence sensors determines mitochondrial oxygen consumption and reactive oxygen species (ROS) generation. The present protocol describes a technique to assess mitochondrial respiratory rates and ROS production in the permeabilized sciatic nerve.