Sign In

35.13 : Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and activates it, resulting in the formation of APCCdc20 and APCCdh1 complexes, respectively. Cdc20 and Cdh1 also help APC to identify its substrates.

APCCdc20 plays an essential role in metaphase to anaphase transition. The sister chromatids are held together by cohesins. The cohesin rings need to be broken by the enzyme separase so that the sister chromatids can move towards the opposite poles during anaphase. However, separase activity is inhibited by the protein, securin. APCCdc20 ubiquitinates securin, leading to its proteasomal degradation. This leads to the subsequent activation of the separase. APCCdc20 also ubiquitinates mitotic cyclins, which in turn inactivates cyclin-dependent kinases or Cdks. The inactivation of Cdks and the subsequent dephosphorylation of Cdk-substrates are necessary for the completion of cell division.

Tags
Anaphase Promoting ComplexAPCCell DivisionMitosisRegulatory ComplexUbiquitin LigaseChromatid SeparationCell Cycle RegulationProtein Degradation

From Chapter 35:

article

Now Playing

35.13 : Anaphase Promoting Complex

Cell Division

2.7K Views

article

35.1 : Mitosis and Cytokinesis

Cell Division

4.4K Views

article

35.2 : Chromosome Duplication

Cell Division

2.0K Views

article

35.3 : Cohesins

Cell Division

1.3K Views

article

35.4 : Condensins

Cell Division

1.3K Views

article

35.5 : The Mitotic Spindle

Cell Division

2.3K Views

article

35.6 : Centrosome Duplication

Cell Division

1.5K Views

article

35.7 : Spindle Assembly

Cell Division

1.3K Views

article

35.8 : Attachment of Sister Chromatids

Cell Division

1.0K Views

article

35.9 : Forces Acting on Chromosomes

Cell Division

1.2K Views

article

35.10 : Separation of Sister Chromatids

Cell Division

1.3K Views

article

35.11 : The Spindle Assembly Checkpoint

Cell Division

1.1K Views

article

35.12 : Anaphase A and B

Cell Division

3.1K Views

article

35.14 : The Contractile Ring

Cell Division

1.4K Views

article

35.15 : Determining the Plane of Cell Division

Cell Division

909 Views

See More

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved