JoVE Logo
Faculty Resource Center

Sign In

Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

DOI :

10.3791/1944-v

April 9th, 2010

April 9th, 2010

14,097 Views

1Department of Chemical and Life Sciences Engineering, Virginia Commonwealth University

Suspension immunocytochemical staining of human pluripotent stem cells (hPSCs) for cell-surface markers (SSEA-3/SSEA-4) was achieved based on use of a self-made cytospin apparatus to create a monolayer of cells for observation and quantification.

Tags

Immunocytochemical Analysis

-- Views

Related Videos

article

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

article

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

article

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

article

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

article

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

article

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

article

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

article

Induction and Analysis of Epithelial to Mesenchymal Transition

article

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

article

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved