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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

DOI :

10.3791/58279-v

October 24th, 2018

October 24th, 2018

7,311 Views

1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere

This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.

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