Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
7.1K Views
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09:34 min
•
August 6th, 2020
DOI :
August 6th, 2020
•Ashutosh Dhingra*1, Joachim Täger*1, Elisangela Bressan*1, Salvador Rodriguez-Nieto1, Manmeet-Sakshi Bedi1, Stefanie Bröer1, Eldem Sadikoglou1, Noémia Fernandes2, Melissa Castillo-Lizardo1, Patrizia Rizzu2, Peter Heutink1,3
1Genome Biology of Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research, University of Tübingen
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Chapters in this video
0:05
Introduction
0:43
New Culture Plate and Tip Loading and Decontamination
1:35
Culture Medium and/or Dissociation Reagent Filling
2:09
Automated Method Execution
2:51
Human Induced Pluripotent Stem Cell (hiPSC) Seeding
4:09
Automated Confluence Assessment
4:47
Medium Change
5:37
Subcultivation
6:42
Automated High Content, High Throughput Imaging
7:14
Results: Representative Automated System-Produced hiPSC-Derived Cortical and Dopaminergic Neuron Characterization
8:58
Conclusion
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