Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
7.1K Views
•
09:34 min
•
August 6th, 2020
DOI :
August 6th, 2020
•Ashutosh Dhingra*1, Joachim Täger*1, Elisangela Bressan*1, Salvador Rodriguez-Nieto1, Manmeet-Sakshi Bedi1, Stefanie Bröer1, Eldem Sadikoglou1, Noémia Fernandes2, Melissa Castillo-Lizardo1, Patrizia Rizzu2, Peter Heutink1,3
1Genome Biology of Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases, German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research, University of Tübingen
Explore More Videos
Chapters in this video
0:05
Introduction
0:43
New Culture Plate and Tip Loading and Decontamination
1:35
Culture Medium and/or Dissociation Reagent Filling
2:09
Automated Method Execution
2:51
Human Induced Pluripotent Stem Cell (hiPSC) Seeding
4:09
Automated Confluence Assessment
4:47
Medium Change
5:37
Subcultivation
6:42
Automated High Content, High Throughput Imaging
7:14
Results: Representative Automated System-Produced hiPSC-Derived Cortical and Dopaminergic Neuron Characterization
8:58
Conclusion
Related Videos
12:10
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
29.8K Views
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
21.4K Views
10:46
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
19.4K Views
13:23
Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
19.9K Views
08:25
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
20.4K Views
07:27
Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
43.5K Views
13:18
Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
21.2K Views
09:43
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
22.4K Views
08:27
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
12.3K Views
10:48
Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions
7.8K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved